Study Of Anti-inflammatory Agents And Antibiotic And The Interaction With DNA By Raman Spectroscopy

Light scattering refers to the phenomenon that light deviates from its original propagating direction and spreads in all directions when passes though the inhomogeneous medium. Raman spectrometry is an analytic method which can get the relevant information so as to realize the qualltative detection because of the changes of the direction and energy of light when the incident light irradiates the sample molecules. As we all know, the phenomenon that the Raman singals of the sample molecules are obviously enhanced when absorbed on the rough surface of some metals and their oxides or some composite materials and semiconductor materials is named as surface-enhanced Raman scattering, and the analytic method based on it is called surface-enhanced Raman spectrometry. Surface-enhanced Raman spectrometry can provide a large number of molecular vibrational structure information in order to determine the types, adsorption orientations and statuses of the adsorbed molecules.The discovery, theory, characteristics and applications of Raman, and the discovery, enhanced mechanism and applications of surface-enhanced Raman spectroscopy(SERS) have been reported in this paper; aspirin, amoxicillin, ibuprofen, and their interaction with DNA have been investigated by Raman spectrometry and surface-enhanced Raman spectrometry. The main research contents are as follows:(1) The methods of synthesis and post-processing of the silver colloidal substrate were discussed. The results showed that the silver colloidal was evengranular and stable. The density was increased and the impurities of the silver colloidal were reduced by centrifugation, therefore the sample molecules were absorbed on the silver colloidal surface more easily and the SERS signals were enhanced.(2) Normal Raman spectroscopy(NRS) and SERS of aspirin and aspirin tablet on the silver colloidal substrate, and SERS and UV of the interaction between aspirin and DNA were investigated. The results showed that, NRS and SERS of aspirin and aspirin tablet were consistent basically; in SERS, aspirin was absorbed on silver colloid through the carboxyl group and the benzene ring perpendicularly; The interaction was mainly caused by the inserting-action mode between aspirin and DNA, and the benzene ring and C=O of aspirin inserted the base pair of the double helix structure of DNA.(3) NRS and SERS of amoxicillin and amoxicillin capsule on the silver colloidal substrate, and fluorescence spectroscopy and SERS of the interaction of amoxicillin and DNA were researched. The results showed that, NRS and SERS of amoxicillin and amoxicillin capsule were consistent basically; in SERS, amoxicillin was absorbed on silver colloid through the nitrogen atom and the benzene ring parallel; The interaction was mainly caused by the inserting-action mode between amoxicillin and DNA, and the benzene ring and the nitrogen atom of amoxicillin inserted the base pair of the double helix structure of DNA.(4) NRS and SERS of ibuprofen and ibuprofen sustained-release capsule on the silver colloidal substrate, and SERS of the interaction of ibuprofen and DNA were studied. The results showed that, NRS and SERS of ibuprofen and ibuprofen sustained-release capsule were consistent basically; in SERS, ibuprofen was absorbed on silver colloid through the carboxyl group and the benzene ring perpendicularly; The interaction was mainly caused by the inserting-action mode between ibuprofen and DNA, and the benzene ring of ibuprofen inserted the base pair of the double helix structure of DNA.