Applied Technical Study Of Crosslinked Chitosan-Queroxy Resin

Flavonoids show clinical effects in the therapy of diseases such as tumor,hyperlipemia, hyperglycemia, etc., due to their antioxidant properties. Separation offlavonoids is promising in industry fields these days.In this study, reflux extraction was applied for the separation of flavonoids fromthree types of samples (buckwheat, golden buckwheat and scutellaria). the extractionyield of buckwheat, golden buckwheat and scutellaria were achieved at95.61%,84.91%,93.04%.. The extractive conditions were investigated including ethanol/water volumeratio, time and temperature. The optimal conditions were85%(v/v) ethanol solution,2.5h,75oC;60%(v/v) ethanol solution,2h and60oC;60%(v/v) ethanol solution,3hand60oC, respectively.Active cross-linked chitosan resin grafted by quercetin (QCCR) was fixed in glasschromatography column. Then the crude extract (0.15mg/mL buckwheat,0.65mg/mLgolden buckwheat or1.5mg/mL scutellaria) was adsorbed by QCCR. The adsorptioncapacities were shown as56.82mg/g,28.65mg/g and46.75mg/g respectively. Thedynamic elution was followed at flow rate of1.0BV/h,1.35BV/h and1.5BV/h. Thepurity was increased to88.74%,85.53%and91.62%with purifying multiple of7.77,4.98and5.22.The partition coefficient were7.25,6.53,6.87by the QCCR. Thefunctional group of QCCR and nuclear parent of flavonoids were closed to each other toform π-π hydrophobic interaction on a cyclic plain, realizing effective separation offlavonoids.