Apoptosis Of Neonatal Rat Cardiomyocytes After Cocultured With Adrenomedullin Gene Transfected Bone Marrow Mesenchymal Stem Cells Under Hypoxia-cultured Conditions

[Aim] To investigate apoptosis of neonatal rat cardiomyocytes after coculture withAdrenomedullin gene transfected bone marrow mesenchymal stem underhypoxia-cultured conditions.[Methods] Rat BMSCs were conventionally harvested by differential adhesion method.The infection efficiency of adenovirus vector to MSCs was tested by x-galstain.Isolation、culture、identification of neonatal rat cardiomyocytes were done.Neonatalrat cardiomyocytes cultured under hypoxia and serum-free conditions for3,6,12,24hrespectively, apoptosis was detected by terminal deoxynucleotidyl transferase-mediateddUTP nick labeling (Tunel). Bone marrow mesenchymal stems and neonatal ratcardiomyocytes were cocultured in transwell system under hypoxia and serum-freeconditions. ADM in culture medium was detected by enzyme-linked immunosorbent(ELISA), apoptosis in neonatal rat cardiomyocytes was detected by Tunel.[Results] Infection rate of MSCs increased with MOI in a dose-dependant manner.When MOI was150, The infection rate was95.4%. After neonatal rat cardiomyocytescultured in hypoxia and serum-free medium, apoptosis increased in a time dependentmanner. Cocultured with MSCs, the apoptosis rate in neonatal rat cardiomyocytes wasdecreased,especially when MSCs were adrenomedullin gene transfected(P＜0.05).[Conclusion] Under hypoxia and serum-free cultured conditions, the apoptosis rate inneonatal rat cardiomyocytes decreased when cocultured with MSCs.And the apoptosisrate even decreased while MSCs were adrenomedullin gene transfected. These resultsmay be related to the increased expression of ADM after hypoxia and adrenomedullingene transfected.