Cytoprotective Effect Of Urinary Trypsin Inhibitor On Astrocytes Injured By Sustained Compression

Objective Cell membrane integrity damage is the primary pathological change observed in traumatic brain injury (TBI), which activates a variety of complicated intercellular and intracellular pathological events and leads to further neural injury. In this study, Using a hydrostatic pressure model of mechanical-like TBI, we first evaluated membrane integrity by determining lactate dehydrogenase (LDH) release to investigate the direct effect of ulinastatin on astrocytes after sustained compression injury. To study the effects of early or delayed ulinastatin administration on astrocytes after sustained compression injury. Methods Astrocytes isolated from1-to2-day-old rat pups cortices were injured by sustained compression. And then injured astrocytes were treated with ulinastatin at different concentrations and times. Subsequently, LDH releases were measured, and astrocytes were examined by transmission electron microscope (TEM). Result When the pressure was0.3Mpa and the duration was5min, there were significant increases in LDH release compared to control samples30min after the compression stimulus, and astrocytes examined by transmission electron microscope displayed structural abnormalites. Early administration (less than2h after injury) of ulinastatin significantly reduced LDH releases at concentrations of1000U/ml and3000U/ml compared to injured samples. Conclusion Appropriate concentrations of ulinastatin may have protective effects on astrocytes injured by sustained compression, and early administration (less than2h after injury) of ulinastatin may result in better outcome compared to delayed administration.