| Background： the Bone marrow mesenchymal stem cells have the differentiation andrepairing features，After interfere with severe acute pancreatic in rats，Observation of thedistribution in the rat various organs and the evaluation of intervention effect on cerebralcapillary leak in rat brain.Purpose：after the bone marrow mesenchymal stem cell treatment for severe acutepancreatitis rats，Observation of the distribution in the rat various organs, discussing the braincapillary leakage effect and mechanism after Bone marrow mesenchymal stem cellsintervention on severe acute pancreatitis rats。Methods: adherent separation and culture of human bone marrow mesenchymal stem cells,90%fusion were digested amplification. Passage third， through cell immune phenotypeidentification，then the suitable bone marrow mesenchymal stem cells were intervented withsevere acute pancreatitis SD rats。200g，72numbes SD male rats were randomly divided intosham operation (SO) group, severe acute pancreatitis (SAP) group and bone marrowmesenchymal stem cells (Bmsc)group, each animal and then divided into6h,12h,24h,threePhases, each phase point8numbers. manufacturing model of severe acute pancreatitis， after1h, according to every one2x106/Bmsc inject into severe acute pancreatitis SD rats throughthe tail vein。In6h,12h,24h respectively obtain the liver, kidney, brain, lung, pancreas,intestinal organ line of fast frozen sections, observation under the fluorescence microscopy,and in the image Pro-Plus software analysis of measuring fluorescence gray valu. Electronmicroscopic observation of severe acute pancreatitis in rat brain tissue microstructure changes,observe the pathological changes of pancreas and detection of serum tumor necrosis factor-A(TNF-a), interleukin-1(IL-1), water channel protein-4(AQP-4) mRNA expression levelchanges. Results：the third bone marrow mesenchymal stem cells having the typical morphology，which conform to Bmsc unique surface antigenic characteristics. mesenchymal stem cells areextracted by green fluorescent protein mice were intervented for severe acute pancreatitis SDrats, observed the distribution in their main organ, pancreas, intestine, kidney, liver, lungsdistribution are more, and the brain distribution are less.the systemic inflammatory response inrat in injury, after24h reaction obviously, MSCs can modulate SAP rats and the systemicinflammatory response, inhibit inflammatory mediator excessive release of anti-inflammatorymediators, promote the secretion, alleviate brain immune damage, relieve cerebral capillaryleakage, relieve cerebral edema, interposition graft MSCs on brain injury have a certaintherapeutic effect. |
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