The Role Of Hydrogen Sulfide In Shock Stage On Severe Scald Rats

Objective To investigate the mechanism of hydrogen sulfide （H₂S） inearly shock of rats after severe burn, to provide new ideas for clinical anti-shocktherapy after severe burns. Methods104male SD rats were randomly divided intonon-burn group and burn group. The burn group was divided into simple burn group,NaHS group and PPG intervention group.30%of Full-thickness burn model wereestablished in each group, to monitor the heart rate at6h,12h,24h and48h after burnby using16-physiological detector through carotid artery catheterization, in normalcontrol group, to direct measure the heart rate through carotid artery. To collect bloodthrough abdominal aorta and test the level of hydrogen sulfide （H₂S）, histamine （HIS）,the content of substance P （SP） in blood serum at each time point, and compared withnon-burn group. Results Serum H₂S concentration in simple burn group at6h and12hwas significantly increased compared with non-burn group, however it was notsignificantly increased at24h and48h after burn, while HIS and SP concentration inserum was persistently increased, abdominal aorta rate at6hours after burn werereduced, and then gradually increased, there are significant differences at each timepoint. Compared simple burn group, NaHS intervention group and PPG interventiongroup, there are significant differences in the level of H₂S, HIS, SP and heart ratevalue. Conclusion in the rat model of burn shock, H₂S could affect serum HIS, SPlevels and blood pressure, H₂S, involved the pathophysiological process of burn shock,and it is expected to become one of the new avenues for anti-shock therapy.