Research Of Cytokines In The Pathogenesis Of The Obstructive Jaundice In Liver Fibrosis

Objective： To investiga te the effect of high mobility group box-1proten(HMGB-1) expression in plasma and hepatic tissue homogenate inrats with obstructive jaundice. Methods： twenty-four male SD ratswere randomly divided into4groups: control group(normal diet), modelgroup(obstructive jaundice). All rats were sacrificed at the end of weeks1,2,3. The levels of a lanine am inotransferase(ALT), aspartate aminotransferase(AST), and the expression of HMGB1in liver tissue weredetermined by immunohistochemical techniques and were analyzedsemiquantitatively by image–analyzing system. The changes ofhepaticm orphology were observed by HE. Results：The liver developedobstructive jaundice in model group at the end of week1. At week2, atweek3, the inflammation aggravated further and the liver developed mildhepatic fibrosis. Compared w ith control group, the levels of ALT, AST,TIDL,BIDL，HMGB-1in model group increased significantly at weeks1,2,3(P <0.05). Conclusion： fibrosis of acute hepatic injury ofobstructive jaundice, HMGB-1formation in liver might be involved inthe pathog HMGB-1plays an important role in the pathogenesis ofobstructive jaundice, HMGB-1level in serum is closely associated withthe aggravation of liver damage and liver fibrosis, which is useful toestimate the disease severity in obstructive jaundice patients.