Molecular Mechanism Of Multi-drug Resistance Of Escherichia Coli And Klebsiella Pneumoniae

Objectives To study the drug resistance of Escherichia coli and Klebsiella pneumoniae, andtesting the carbapene-mase and Resistance gene. analysis of bacteria resistance gene spreadrelationship the by PFGE, in order to provid clinical of the basis for rational use of antimicrobial.Methods These isolates were identified by the automatic VITEK2system and theircarbapenem-resistant Escherichia coli and Klebsiella pneumoniae was tested by microdilution.car-bapenem enzyme was tested by Hodge. carbapenem class B enzymes (metallo) was tested byEDTA/IMP, EDTA/CAZ complex paper and imipenem and ceftazidime for jointexperiments.positive and negative of Carbapenems enzyme antibiotics statistics analysis of16kindof MIC. The polymerase chain reaction (PCR) was got of carbapenem-resistant gene andsequenced to identify the types of genes. The broth culture and then diluted to0.5wheat type ofbacteria was embedded in plastic block of low melting,and with the appropriate endonuclease insitu digestion of the bacterial chromosome,and to pulsed-field gel electrophoresis analysis ofgenetic between relationship among strains of bacteria.Results8strains of Escherichia coli and28strains of Klebsiella pneumoniae were drugresistance the cephalosporins, carbapenems and fluoroquinolones.5strains of Klebsiellapneumoniae were sensitived aminoglycosides。by Hodge testing were screen8strains ofEscherichia coli and28strains of Klebsiella pneumoniae, a total of30strains for positive,3strains of Escherichia coli, the positive rate was37.5%.26strains of Klebsiella pneumoniae forpositive, the positive rate was85.7%. Synergy experiments to test36strains of bacteria, onlyfounds a metal enzyme-positive strains, belonging the Klebsiella pneumoniae, A method wasdetecting of metal enzyme better than the B methods. There were4strains of KPC gene among8strains of Escherichia coli, the positive rate of50%;28strains of Klebsiella pneumoniae wereoccurred in the26positive strains, the positive rate of92.8%.36strains of E. coli and Klebsiellapneumoniae were not found in VIM and IMP genes.36bacterial PFGE homologous analysisshowed that36Klebsiella pneumoniae and Escherichia coli for the polyclonal form, with a highdegree of differentiation. In the sub-type, a total of21different forms of cloning, three cloning appeared spread, divided into A-type bacteria involved5, B-type involves three types of bacteria,C-type involved in four, three of which appear cloning spread. accounted for58.3%of the studiedstrains. A clone with four of five strains from the ICU,1strains from respiratory ward, number ofstrain K20strains for the first clone, live ICU ward with K18, K21, K19numbers of patients withthe cross and hospital stay. B involves three cloning of bacterial, first strains of K11isolated inthe respiratory ward after ward into the ICU. There are three strains of bacteria clone C, E1as thefirst strains isolated in general surgery. Number of E3isolated in the ICU, E1lived some time inthe ICU into the general ward, there are cross-hospital stay, while the E1and E9did not cross-hospital stay. A cloned and B cloned and C cloned repeated transmission, amikacin MIC wasvalues rising.Conclusion The bacteria resistant to carbapenems antibiotics mainly spread in intensive careunit and respiratory department.The reason for resistance is mainly KPCgene,but VIM and IMPgene could not be ruled out. The spread of bacteria clone enhances resistance of bacteria toantibiotic.