Expression Of CD147, PCNA, VEGF, MMPs And Clinical Significance In Giant Cell Tumor Of Bones

Giant cell tumor of bone (GCT) is a potentially malignant tumor. The incidence of this tumor in China is2-3times more common than in the western countries. Local aggressive growth and post-operative recurrence scale is as high as40%-50%. The pathological classification and image credits type of GCT cannot be precisely predicted with its clinical behavior. So, it’s very important to consider other factors,which relate with the GCT’s biological behavior and prognosis.Extracellular MMP inducer (EMMPRIN, CD147) is a member of immune-globulin (Ig) super family, and a cell surface glycoprotein can be expressed through numerous cell types. CD147is a multifunctional protein, which plays an important role in the development, reproductive physiology, immune regulation and so on. EMMPRIN in tumor invasiveness has been confirmed immune-histochemically in several types of cancer cells, including astrocytomas, myeloma, and melanomas. It is involved in the origin and development of the tumor through several tumorigenic mechanisms:①Promoting tumor Invasion and metastasis.②Inducing tumor angiogenesis.③Promoting tumor Anchorage independent growth. Matrix metalloproteinases (MMPs) and vascular endothelial growth factor (VEGF) are exist in a variety of human tumor tissues and play an important role in the invasion and metastasis and angiogenesis of tumor. Matrix metalloproteinases (MMPs) comprises of more than20enzymes, which degrade the basement membrane and extra-cellular matrix (ECM) components in numerous physiological and pathological situations. An increased MMP activity is considered as an important contributor to tumor invasion and metastasis, which destroys ECM components. These potentiate tumor cell formation, migration, intravasation, and extravasation. VEGF is the most extensively studied angiogenic factor. It has been related with tumor progression, metastasis, and prognosis. Proliferating cell nuclear antigens (PCNA) represents cell proliferation function effectively and have been widely used in research of tumor proliferation activity. PCNA is an important index which could evaluate the proliferation state and malignant potential of tumor. In this study, we have examined the immunohistochemical staining patterns in a series of GCT patients, with the objective of elucidating their prognostic role and their correlation with CD147, MMPs, VEGF, PCNA, and CD34.Objective To study the correlation between CD147and PCNA、VEGF、MMPs expression in giant cell tumor of bones and its relationship to clinical pathology and prognosis, and find a new indicator in the prognosis of GCT.Method68patients (32males and36females, aging12-58years, average34years) with GCT were obtained from the Department of Orthopaedics, Xijing Hospital, Xi’an, Shaanxi, P.R China. All the specimens were fixed in10%neutral buffered formalin. These were subsequently embedded in the paraffin. The paraffin-embedded tissues were cut at4μm. To detect the expression level of CD147、PCNA、VEGF、MMPs in68cases of giant cell tumor of bones by using S-P immunohisochemistry, and analyze its relationship between each other and with the clinical pathology information.Results The significant difference of expression level of CD147、MMPs was found in the cases of GCT with Jaffe grading prognosis(P<0.05), but not with the patient’s age, sex. Expression of CD147was positively correlated to MMP-2, VEGF, MVD, PCNA (r=0.271,P=0.025;r=0.411,P=0.000;r=0.872,P=0.000; r=0.394,P=0.001).Conclusion The expression level of CD147in giant cell tumor of bone is correlate with canceration and relapse, and there was positive correlation between the expressions of CD147and MMP-9、VEGF、MVD、PCNA, and CD147might be an important indicator in evaluating the malignant degree and prognosis of giant cell tumor of bone, and may be the new targets for chemopreventive strategies.