Expression Of Stem Cell Factor SOX2in Gastric Cancer And Its Molecular Mechanism Research

Objective:To study the expression and clinical significance of SOX2in gastric cancer and toinvestigate its molecular mechanism research.Methods:1. Polymerase chain reaction (PCR) and Western-bloting methods were used to detectthe expression of SOX2in human gastric epithelial cell and the different malignant degreeof gastric cancer cells.2. Polymerase chain reaction (PCR) and Western-bloting methods were used to detectthe expression of SOX2in normal human gastri mucosal, paraneoplastic tissues andgastric cancer tissues.3. Methylation-specific polymerase chain reaction (MS-PCR) method was used todetect the SOX2DNA methylation level of gastric cancer cells.4. Survival rate of gastric carcinoma cell with treatment of different concentration andtime of5-Aza-CdR was measured using MTT metabolic viability assay（P＜0.05）.5. Polymerase chain reaction (PCR)、Western-bloting and Transwell cell invasive trialin vitro methods were used to detect the expression and invasion of SOX2in gastric cancercells following treatment to5-Aza-CdR.Results:1. The mRNA and protein expression of SOX2poor expressed in gastric cancer cellsand tissues, and the positive particle decreased as the degree of differentiation. while thedifference was significant(P<0.05)in the expression of SOX2in cells.2. The expression of SOX2in mRNA and protein were found significant differences(P＜0.05)in differentiated degree and tumor invasion in gastric cancer tissues, while the difference was no significant (P>0.05) in sex、age、Clinical stages and lymphatic metastasis. The resultdemonstrate that the expression of SOX2following the decline of malignant degree grades.3. The methylation staus of SOX2promoter region in gastric cancer cells, whileunmethylation staus of that in human gastric epithelial cell.4. The proliferation of gastric cancer cells was inhibited in a dose-dependent andtime-dependent manner after the treatment of5-Aza-CdR.5. The mRNA and protein expression of SOX2was restored in gastric cancer cellsfollowing treatment to5-Aza-CdR(P＜0.05), and the invasive ability of gastric cancercells was decreased(P＜0.05).Conclusion:1. The mRNA and protein poor expression of SOX2in gastric cancer cells and tissues.Itwas found significant differences in differentiated degree and tumor invasion in gastriccancer tissues.2. The methylation staus of SOX2promoter region in gastric cancer cells, and5-Aza-CdR can restore the expression of SOX2in gastric cancer cells, lead to the invasiveability of gastric cancer cells was decreased.