Expression Of MACC1in Rectal Adenocarcinoma And Its Correlation With Epithelial-mesenchymal Transition

AIM: To investigate the expression of MACC1in rectal adenocarcinoma and therelationship between MACC1and epithelial mesenchymal transition of rectaladenocarcinoma.Methods: Reverse transcriptase polymerase chain reaction (RT-PCR) was used todetermine the expression of MACC1， E-cadherin and vimentin mRNA in30pairs ofrectal adenocarcinom and the adjacent normal tissues. Immunohistochemistry wereused to examine the expression of MACC1， E-cadherin and vimentin protein in90specimens of rectal adenocarcinoma and30specimens of adjacent and analyse therelationship of them.Results: The RT-PCR examination showed that the relative expression level in rectaladenocarcinoma tissues were significant higer than that in normal rectum tissues(0.535±0.310vs0.373±0.190, P<0.05); By Immunohistochemistry examination, thepositive rates of MACC1in rectal adenocarcinoma tissues were also significantlyhigher than that in the normal tissues(86.7%vs36.7%, P<0.01). By univariate analysis,the overexprossion of MACC1protein was correlated with the clinical stage anddistant metastasis(P<0.05)， but not to patients age, sex, tumour size,Differentiation degree, lymph node metastasis(all P>0.05). Cox regressionmultivariate analysis showed that MACC1positive expression is an independentfactor for the prognosis of rectal adenocarcinoma. The Pearson’s correlation analysisdemonstrated that MACC1expression was negatively correlated with E-cadherinexpression(r=-0.326；P=0.002)， while it was positive correlation with Vimentinexpression(r=0.284；P=0.007).Conclusions: MACC1is up-regulated expression in rectal adenocarcinoma，and isclosely related to tumor metastasis, it is an independent factor for the prognosis ofrectal adenocarcinoma. MACC1may be involved in epithelial mesenchymal transition of rectal adenocarcinoma.