The Relationship Between The Regulation On Osteoblast Functional Protein And The Activity Of PPARγ Affected By Metformin

Objective1.Observe the effect of regulating the activity of PPARγ on the expression of osteoblastfunctional protein.2. Observe the effect of metformin on the expression of osteoblast functional protein,and the relationship between this effect and the activity of PPARγ.3.Analyze whether the effect of meformin on osteoblast mentioned above isindependent of AMPK.Methods1.Inhibit or activate the activity of PPARγ in osteoblast with inhibitor (GW9662) oragonist (Pioglitazone Hydrochloride) of PPARγ respectively for3weeks, and observe theexpression of functional protein (BMP-2, ALP and OCN), p-AMPK and PPARγ.2. Intervene the osteoblast with metformin for3weeks to observe the expression offunctional protein (BMP-2, ALP and OCN), p-AMPK and PPARγ.3.Inhibit the activity of AMPK in osteoblast by Compound C, and then intervene theosteoblast with metformin for3weeks. Observe the expression of functional protein(BMP-2, ALP and OCN), p-AMPK and PPARγ.4.Inhibit the activity of AMPK in osteoblast continuously and intervene it withmetformin for3weeks. Observe the effect of metformin on the inhibition of osteoblastfunctional protein(BMP-2, ALP and OCN) mediated by Pioglitazone Hydrochloride, andthe expression of p-AMPK and PPARγ.Results1. Compared with the control group, the expression of functional protein and p-AMPKcould be promoted by activating PPARγ, and could be reduced by inhibiting PPARγ.2.Compared with the control group, metformin could promote the expression offunctional proteinand p-AMPK, but inhibit the expression and the activity of PPARγ. 3.The effect of metformin which promoting the expression of functional protein andinhibiting the expression of PPARγ could be weaken other than disappearrent wheninhibiting the activity of AMPK.4.Metformin could still improve the inhibition of functional protein mediatied byPioglitazone Hydrochloride, and could downregulate the expression of PPARγ.ConclusionsThe promotion of osteoblast functional protein mediated by metformin may beindependent of AMPK,and may be associated with the activity of PPARγ.