Proteomic Analysis Of The Ultra-short Bowel Syndrome In Rats’ Compensatory Colon

Background:Short bowel syndrome (SBS) is caused by chronic malabsorptionsyndrome due to extensive small bowel resection, when remnant small bowel (RSB)<30cm, is called ultra-short bowel syndrome (USBS).USBS may have a severemalabsorption and malnutrition, currently still did not have a satisfactory treatmentcurrently except parenteral nutrition (PN). Patients have to depend on PN for wholelifetimes, which lead heavy burden to the social and family. Some of the previousstudies had shown that when the USBS happened, the colon could also compensate.How to promote colon compensatory and enhance the absorption features of colon arethe key features to improve the quality of the USBS patients’ survive, and also is one ofthe treatment direction of USBS, but the mechanism of colon compensatory mechanismis still unclear. Our previous study confirmed the ultrastructure and the absorptionfeatures of the USBS rat colon had a obvious compensatory changes. By the time, wehave identified10kinds of associated colon compensatory protein such as proteindisulfide isomerase A3(PDIA3), pyruvate kinase (PK) with Proteomics. By furtherstudying the colon compensatory differences in protein expressions, and suggesting thepossible regulation of the USBS colon compensatory protein, so as to elucidate themolecular mechanisms of colon compensatory and presume the colon compensatorypromote molecules of have a great significance value, and it’s worthy for further study.AIM: To establish a rat model of ultra-short bowel syndrome with enteral nutritionsupport and use proteomics technology to study the differential protein expression incompensatory colon, so as to provide an experiment basis to clarify the coloncompensatory mechanisms to support the research on compensatory drugs development.Methods:30male SD rats, weighing250-300g, were randomly divided into threegroups:(1) Ultra-short bowel group (n=10): surgical removal of90%to95%of thesmall intestine;(2) Sham operation group (n=10): about12cm from the ileocecal valveof the small intestine underwent transection and anastomosis. The two groups of rats were fed by enteral nutrition.(3) Control group (n=10): normal rats with the samemethod as the above two groups and nutrition feeding. Postoperative enteral nutrition for21days, the three groups of rat colonic mucosa epithelium was extracted as specimens.By using the application of two-dimensional gel electrophoresis (2-DE) massspectrometry (MS) proteomic analysis, protein expression in the three groups werecompared in the rat colon and to identify differentially expressed proteins, to understandthe function and classification of these proteins by querying the relevant biologicalinformation database, and preliminary analysis of the possible role in coloncompensatory.Results:(1) We established3gel maps from three experimental groups by2-DEtechnology, and then selected the expression proteins which had more than two timesdifferences. By comparison and analysis:1) Between Ultra-short bowel group andNormal group, that had46differential protein spots, including41up-regulated spots,5down-regulated spots, with only35spots appeared in the ultra-short bowel group.2) InUltra-short bowel group and the Sham group, there were30differential protein spots,with the up-regulation of20spots and down-regulation of10spots, within them sixspots only appeared in the ultra-short bowel group.3) By Comparing the above two setsof expressed proteins spots, we differentiated totally eight common protein spots, ofwhich seven were up-regulated more than2times, one down-regulated greater than2times, only five in the ultra-short bowel group.(2) Four common up-regulated proteinspots were selected for the MS processing, and four kinds of difference proteins wereidentified: Aldehyde dehydrogenase-2、Major vault protein、Wnt2protein、Agr2protein.Conclusion:We identified four proteins that played an important role in coloniccompensation: Aldehyde dehydrogenase-2、Major vault protein、Wnt2protein、Agr2protein. These proteins may take part in the oxidation reduction reactions, cell growthand differentiation, anti-oxidative stress, signaling pathways, etc. in the rats’compensatory colon.