Analysis Of Mirobial Diversity Of The Banana Soil In Hainan Fushan

Banana wilt disease is one of the extremely devastating diseases in banana production. Biological control is the most effective and environmental way for banana wilt. Biological control of foreign colonization of antagonistic bacteria worked as important means of the continuing fight against soil-borne diseases.But the soil assumed the symbiosis place involved with varieties of microbe.There was close relationship between the field control effect of foreign antagonistic bacteria and the soil of microbial environment. Soil samples were gotten from the normal and diseased banana lands in Fushan town Chengmai county Hainan province. The microbial number diversity was analyzed. Morever,the 16SrDNA Library and ITS library were constructed in order to compare the difference between the two libraries and to prove the impact of banana wilt on the microbial number diversity. References could be provided to the biological control of banana wilt eventually. The research results were as follows:The numbers of soil bacteria and fungi in Fushang banana lands was assayed by the way of plate counting method. It revealed that the number of bacteria added up to the peak in the seedling stage and then decreased. While those varieties of fungi increased gradually during the banana growing process. Compared with the normal banana lands, the quantities of fungi in the banana wilt lands increased obviously,while, bacteria decreased in the bud stage.Analysis of the 16SrDNA cloning libraries showed that:the 16SrDNA library of the normal banana lands exhibited better diversity and covering six phylums. The dominant bacteria were Proteobacteria. Others were Firmicutes, Actinobacteria, Acidobacteria, Cyanophyta, Nitrospira etc. In contrast, the number of Proteobacteria in the wilt disease banana lands was much smaller and the main bacterium communities were Firmicutes and Actinobacteria. It showed that Proteobacteria was in inferior position but Firmicutes and Actinobacteria reproduced effectively in the ecological competition with the Fusarium which caused the banana wilt diseases.Submited sequence data to GenBank., they have provided GeneBank accession numbers for nucleotide sequences:HM131954-HM131977.Comparative analysis of the ITS gene libraries indicated that the types of soil fungi were less than that of the soil bacteria. Cladosporium sp. and Stcnella sp. belong to Adelomycete sp. were 50% in the normal banana lands with some other fungi genera.What is more, a small quantities of Fusarium sp. belong to.Adelomycete s was found. Sequences similar to that of cilia animal and uncultured endophytes were found in ITS gene libraries of the normal lands additionally. In the diseased lands, the main fungi were the Fusarium sp. which led to the wilt disease. Few of the positive clones were Cladosporium sp., Stcnella sp., Urostyla grandis, Heterochaetesp., Alternaria ect. Fusarium sp.was in dominant position in diseased lands though there was few Basidiomycota. It indicated that the Basidiomycota fungi may be more effective to the disease control and soil improvement.Submited sequence data to GenBank., they have provided GeneBank accession numbers for nucleotide sequences:HM131978-HM132004.In summary, banana wilt disease affects the microbial environment in the soil to a certain extent. It also decreases the microbial community diversity in the banana wilt disease soil significantly compared with normal lands.