Analysis Of Differentially Expressed Proteins In Self-rooting Juvenile Type Clones And Their Donor Mature Type Clones From Rubber Latex (Hevea Brasiliensis)

Natural rubber is an important strategic materials and industrial raw materials, play a very important role in national economic development. Under the limited rubber planting area,it’s urgency to improve the coverage hight yield planting material. As a new planting material, self-rooting juvenile type rubber trees clones has a fast growth, high volume production of latex, and a strong resistance characteristics. While the research of yielding mechanism in self-rooting juvenile type of rubber tree clones is little, and now only some morphological and physiological studies, such as the rubber tree latex output level relies tolatex physiological relevance, but lack of directly evidence on molecular biology.Conbinding with our laboratory epigenetic studies,differences in transcriptomics and gene clone technology,compare with the complementary application of proteomics, provides us a powerful tool to study high yield mechanism of self-rooting juvenile type rubber tree clones in systems biology. We use high-throughput proteomics technology platform to analysis differentially expressed proteins between the self-rooting juvenile type and their donor mature type rubber tree clones, revealed the key functional proteins in high yield mechanism of self-rooting juvenile type rubber tree clones. This thesis work consists of two parts:1. Physiological and biochemical indexes:measuring the latex physiological and biochemical indexes between the rubber trees self-rooting juvenile type clones and their donor mature type clones, including the dry rubber content (self-rooting juvenile type clones: 33.05%,their donor mature type clones:30.16%), PH value (self-rooting juvenile type clones: 6.94,their donor mature type clones:6.77), sulfur content (self-rooting juvenile type clones: 0.54 mM/L, their donor mature type clones:0.44mM/L), inorganic phosphorus (self-rooting juvenile type clones:17.35 mM/L, their donor mature type clones:11.65 mM/L), sucrose content (self-rooting juvenile type clones:2.4 mM/L, their donor mature type clones:2.9 mM/L), sucrose transferase extraction and enzyme activity determination (self-rooting juvenile type clones:0.457U their donor mature type clones:0.636U).2. Establishment and improvement of Proteomics technology platform, the basis of the subsequent studies.Improvement and optimization of rubber latex protein extraction and electrophoresis conditions, got the high-resolution, high reproducibility, comparability and stability of two-dimensional gel electrophoresis maps. Through proteomic analysis of latex found 37 differentially expressed proteins. By MALDI-TOF-MS identified 27 positive results, which 24 proteins was successfully identified.10 proteins may be associated with the high yield Mechanism. The main function of these proteins were concentrated in the Carbohydrate and energy metaboism, Secondary metaboism, Signal translocation, Transcriptional regulation-related, Protein synthase,assemthy and degradation, transport, Nucleoside acid process, Lipid metaboism, unknown function proteins。...