Proteomics Analysis Of Mulberry Leaves

As the largest silk industry country in the world, China has a long history of mulberry planting and silkworm feeding. As a result of mulberry sericulture and mulberry planting cultivation from all parts of our country, a lot of mulberry varieties were produced. In statistics, there are more than2600species of mulberry across the country which accounts for the biggest part of the world. At the same time, with our laboratory finished the whole genome sequencing of mulberry CHUAN (Morus notabilis Schneid), mulberry proteomics research are more urgently and necessarily needed.In this study, we used differential proteomics approach to research mulberries, differential Proteomics is a study approach which using the protein composition changes caused by meaningful difference factors to find a difference kinds of proteins or to look for differences in different varieties.In order to understand the difference of protein expression and varieties characters between different mulberry varieties, we selected51varieties of mulberry mature leaves as experiment materials. First of all, we checked51varieties of mulberry leaves’ protein content, the highest one is DAYE, YU711is the lowest. Then, we used SDS-PAGE to check the protein expression in those51samples. In seven mulberry species (5801, XIANG7920, JIALING30, HONGGUO2, YU2, YU151, YU711), we observed special small molecular weight proteins expression which are different from the other44varieties. In order to identify the special expression in the7varieties, we chose the most representative JIALING30and HONGGUO2mature leaves as materials, HU32as a control, with two-dimensional electrophoresis and MALDI-TOF-TOF mass spectrometry identification technique, we identified two proteins which are only expressed in JIALING30:RNA polymerase Ⅱ carboxyl-terminal domain (CTD) phosphorylase.We discussed the function of this enzyme in mulberry.