Studys On The Proteome Related To Pistillate Flower Development In Mulberry

Fruit mulberry cultivation has become a new field of the modern development ofsericulture industry, the growth and development of mulberry’s pistillate flower andfruit have already been gained more and more attention. In order to explore themolecular mechanism of pistillate flowering development and regulation, the proteomeexpression was investigated by using pistillate flowers from different florescence andposition, pistillate flowers after pollination and ripe fruit of mulberry varieties named“Da10”and “SG01”. Through these studies, we realized the function of different proteincomponents connected with the molecular genetics of pistillate flowers. The resultswere as follows:(1) Based on the SDS-PAGE technology and mass spectrometry, the differentbands protein components were detected from different florescence of mulberry’spistillate flower of “Da10”. In the further study with IPG-IEF/SDS-PAGE analysis, wefound the general numbers of protein increased and then decreased. And the quantity ofprotein components which were the utmost luxuriance of pistillate flowers achieved471±4, while starting and termination respectively achieves450±15and446±14. Result21protein components which were identified from25spots was clearly isolated in2-DE of pistillate flowers by using mass spectrometry techniques. Much has beenlearned from classical biochemical approaches about the expression of transcription andtranslation, cell metabolism, oxidation-reduction, cellulosity, and plant defenses wereclosely related to the normal blooming of pistillate flower in mulberry.(2) We chose the two-dimensional electrophoresis and mass spectrometry to isolateand identify the protein components from the style (with the stigma) and the ovary wasin its utmost luxuriance of pistillate flower of “Da10”. The results showed that theproteome from different position of pistillate flower had significant differentialexpression.28better separations of differentially expressed protein spots were selectedto be analyzed by mass spectrometry from2-DE, and26of them were successfully identified. Much has been learned from classical biochemical approaches about theseprotein components mainly related to plant stress responses, secondary metabolites,oxidation-reduction reaction, and biosynthesis and anabolism of protein, The resultsshowed that differentially expressed of the protein components were closely related topollen germination, growth of the pollen tube and the intrusion of other xenobiotic.(3) Moreover, we chose the two-dimensional electrophoresis to isolate the proteincomponents from pistillate flowering and had already pollinated and ripe fruit of “Da10” and “SG01”. The proteome from different varieties of pistillate flowers with alreadypollinated had significant differential expression.9protein components were identifiedfrom the expression difference in13proteins reached more than two times in differentvarieties by mass spectrometry. Much has been learned from classical biochemicalapproaches about these protein components mainly related to the synthesis andcatabolism of protein, amino acid metabolism, carbohydrate metabolism and plant stressresistances. Secondly, we found the proteome from different varieties of ripe fruit hadsignificant differential expression too.5protein components were identified from theexpression difference in20proteins by using mass spectrometry. Much has been learnedfrom bioinformation analysis approaches about these protein components mainly relatedto plant responses, cell composition, protein synthesis and catabolism. The resultsindicated that the protein components were significant different in varieties, and thedifferences get more obvious after pistillate flowers developmental into fruit.Above all, the development of pistillate flower involved in kinds of proteins whichrelated to a lot of phenomenon of life, gene transcription and translation, both matterand energy metabolism, oxidation-reduction reaction, cell composition and plantresponses. The discovery was fully meaningful for development and regulation, and it’sevident to me that it provided new information about studying the molecular breeding ofmulberry.