The Expression Patterns And Transgenetic Research Of AmRRM,AmSTZF, AmZFPG And AmCaM From Ammopiptanthus Mongolicus

The expression patterns of four genes which are RNA banding protein gene AmRRM, zinc finger protein genes AmZFPG and AmSTZF, and calmodulin gene AmCaM from Ammopiptanthus mongolicus were carried out by real-time PCR. Results indicated that both AmRRM and AmSTZF were induced by cold, drought, salt and heat stresses, while AmZFPG were induced by cold, drought and salt stresses, but for AmCaM, only cold and heat stresses can improve its expression levels. As a result, these four genes may involve in the course of signal transduction, and play important roles in response to abiosis stresses in Ammopiptanthus mongolicus. Overexpression of AmRRM and AmZFPG in transgenetic tobacco resulted in enhanced tolerances to cold, drought and salt stresses, while AmSTZF to drought and salt stresses. For AmCaM, the transgenetic E.coli exhibited higher tolerance to cold and heat stresses comparing with the wild E.coli. Taken together, these results demonstrate that AmRRM, AmZFPG, AmSTZF and AmCaM are exactly involved in the defensive response of Ammopiptanthus mongolicus to abiotic stresses and therefore valuable genes for improving plant abiotic stress tolerances.