Detection And Analysis Of Resistance And Resistance Gene Of E.coli Isolated From Part Of Dairy Farms In Heilongjiang Province

E.coli is the environment and animal body often in bacteria, pathogenic E.coli can cause calf diarrheaand udder infection such as cystitis. Carried out the bovine source and environmental E.coli resistancesurvey to understand dairy farms commonly used antibiotics, monitoring bovine and environment E.coliresistance development dynamics. This study is conducive to master the spread of resistant bacteria ofanimal origin, guiding significance for the prevention of bacterial disease outbreak. In this study, a total oftwo parts:(A) Using the disk diffusion method recommended by the WHO, NCCLS (2005) criteria to detect the72isolated from E.coli scale dairy farms (cattle37, Environmental35)15kinds of commonly usedantimicrobial drugs resistance, analysis and comparison of the herd and environmental E.coli to variousantimicrobial resistant and multidrug-resistant cattle and environmental isolates of Escherichia coliresistance rate to commonly used antibiotics tetracycline, sulfa The highest antibacterial drugs, polymyxinB antimicrobial All sensitive.(B) The herd and environmental isolates of E.coli using conventional PCR to detect different kinds ofmechanisms of resistance genes. GyrA, GyrB and Parc gene for quinolone resistance mechanisms ofresistance detection cattle E.coli the three gene amplification positive detection rates were27.0%,94.6%and97.3%;5herds resistant quinolones E.coli positive rate is80%; corresponding three genes in35environmental isolates of E.coli detection rate of17.1%,97.1%and94.3%, respectively. For theaminoglycosides kanamycin resistance phosphate transferase Aph(3’)-II gene detection, gene resistantstrains in the herd and environmental positive rate of71.4%and66.7%, respectively; aminoglycoside thetransferase enzyme aadA1and aadB of gene positive rate were57.1%and42.8%, while the theenvironment resistant strains were detected in33.3%and100%resistant strains in cattle. Chloramphenicolresistance mechanisms detected the cmlA and Flor genes in E.coli and the herd corresponding genedetected positive rates were2.7%and8.1%, in the environment of E.coli detection rate of0. Sulfa drugresistance mechanisms to detect the sul2gene, the positive rate of detection in herds and environmentalresistant strains were27.3%and54.5%. The tetracycline resistance Teta gene detection rate is0.In summary, the Heilongjiang dairy farm environment and cattle separation of E.coli produce a certainresistance and corresponding resistance gene detected. Experiments confirmed the the cattle isolatesresistant to aminoglycosides plasmid can pass resistance. The emergence of resistant strains is bound to theclinical treatment difficult. The study provides some references for selection of effective drugs forveterinary clinical and control of bacterial resistance.