| Manganese （Mn） is an essential trace element, which is necessary for biological developmentand normal function. However, with the wide range of usage of Mn in industry and agriculture inrecent years, excessive Mn exposure leads to the accumulation and intoxication in animals, affectsbiological normal function. So far, the researches on toxicity of Mn focus on nervous system andgenital system, while the reports on immune system were seldom, even of poultry immune cells.The aim of this study was to investigate the effect of manganese on cytokine and inflammatoryfactors mRNA expressions of chicken spleen lymphocytes in vitro. In this study, lymphocyteswere incubated in culture medium in the absence or presence of MnCl-42(2×10-4×10-4,6×10-4and8×10-4mmol·L-1) for12,24,36and48h, respectively. Then, the mRNA levels of inflammatoryfactors （TNF-α, NF-κB, iNOS, COX-2and PTGES） cytokines and (IL-2, IL-4, IL-6, IL-12β, IL-17and IFN-γ) were examined by real-time PCR. The results showed:（1）Effect of manganese on TNF-α, NF-κB, iNOS, COX-2and PTGES mRNA expressions ofchicken spleen lymphocytes increased firstly, then decreased with dosage. It demonstrated that Mnpromoted inflammatory reactions. But excessive Mn impaired the immune function of chickenspleen lymphocyte.（2）Effect of manganese on IL-2, IL-4, IL-6, IL-12β and IL-17mRNA expressions of chickenspleen lymphocytes decreased except that the mRNA level of IFN-γ increased with increaseddosage.It demonstrated that excessive Mn induced the immune function was also suppressed,disturbed immune responses and induced inflammation reactions. The research would providesome basic data for the further research on immunotoxicity of Mn. However, the mechanism ofMn toxicity in poultry immune needs to be further researches. |
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