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The Research On Slow Release Oxygen Fertilizer Remove Flooded Stress In Forest Tree Seedings

Posted on:2014-01-20Degree:MasterType:Thesis
Country:ChinaCandidate:L WangFull Text:PDF
GTID:2233330398457187Subject:Biochemistry and Molecular Biology
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Flooding Stress is one extremely major natural disaster in our country, Huanghuai Plain and the middle and lower reaches of the Yangtze River are the most serious flooding areas. Flooding Stress cause excessive water in soil, the soil moisture and oxygen content exist mutually restricted relationship, excessive water replace the oxygen in the soil and make the lack of oxygen, reduce oxygen concentration in plant rhizosphere, cause the hypoxic stress and anaerobic stress, affect the normal physiological metabolism and growth of plant. At present, people mainly adopt water conservancy、drainage in time、reasonable agricultural cultivation measures、 Submergence-tolerant gene cloning to reduce economic losses. However, they can not solve the problem of hypoxia caused by flooding. In this paper, trying to remove water stress of plants using the slow-release oxygen fertilizer from its source, to reduce economic losses caused by natural disasters. The aim was to study the effect of slow-release oxygen fertilizer on the anti-waterlogging mechanism of plants, and provide theoretical basis and innovative technology for efficient and environmental slow-release fertilizer. There are very important practical significances for Scientific configuration of the landscape and Solving the utilization of flood waterlogging land.In this paper, Macropanax rosthornii (Harms) C. Y. Wu ex Hoo was chosen as experimental material, water was selected as the stress factor, in artificial simulation of flooding stress, the "double sets of pots of soil cultivation experiment, the relationship between slow-release oxygen fertilizer and flooding stress as well as the mechanisms for the protection of plants from the anoxic effects of flooding stress were systemically studied using the physiological and biochemical methods. The results were as follows:1. The tablet slow-release oxygen fertilizer is successful prepared by the tabletting method. The slow-release oxygen fertilizer use calcium peroxide as the core, sodium bentonite as filling materials, polyvinyl formal as adhesive. I examined the types of bentonite, the ratio of sodium bentonite and calcium peroxide, the amount of polyvinyl formal to affect the preparation of the slow-release oxygen fertilizer through the single factor experiment. We can draw a best conclusion that:Sodium bentonite as filling materials, the ratio of sodium bentonite and calcium peroxide is12:5, the amount of polyvinyl formal is15%. 2. The changes of pH and oxygen of the slow-release oxygen fertilizer are preliminary determined though the water immersion experiment. Showing that the pH values of the slow-release fertilizer in hydroponics always sustain a neutral range, which will not change the soil micro-environment, combined with the regulating ability of the soil environment, much less cause hardened soil, then affect normal growth of the plant. In addition, the slow-release oxygen fertilizer can release oxygen for around30days, which will be able to slowly release oxygen, and provide oxygen to the flooding plants, do not appear to release suddenly and largely and rapid decline.3. Applying the slow-release oxygen fertilizer is helpful to the accumulation of chlorophyll content in Macropanax rosthornii (Harms) C. Y. Wu ex Hoo leaves under regular growth, when applying the slow-release fertilizer reach the twentith, twenty-fifth, thirtieth day, the chlorophyll content2.2,2.7,2.8times compared to CK; antioxidant enzyme activity、content of the malondialdehyde (MDA) and the relative permeability of membrane are more stable than CK; root activity greatly increased.4. It is not harmful to the growth of Macropanax rosthornii (Harms) C. Y. Wu ex Hoo under Waterlogged stress. However, under Waterlogged stress, applying slow-release oxygen fertilizer is more helpful to the growth of Macropanax rosthornii (Harms) C. Y.5. When the water stress reach the tenth day, the chlorophyll content and root activity of Macropanax rosthornii (Harms) C. Y. Wu ex Hoo leaves decrease by58.2%and86%, the relative permeability of membrane and malondialdehyde (MDA) of Macropanax rosthornii (Harms) C. Y. Wu ex Hoo leaves increase by250%and590%; Superoxide dismutase (SOD) and peroxidase (POD) activity plung sharply, completely lose activity about13days. Compared to CK, the change of physiological and biochemical indexes in F treatment:when the Water stress reach the tenth day, the chlorophyll content and root activity of Macropanax rosthornii (Harms) C. Y. Wu ex Hoo leaves decrease by10%and25%, the relative permeability of membrane and MDA of Macropanax rosthornii (Harms) C. Y. Wu ex Hoo leaves increase by190%and220%; SOD and POD activity rose firstly then fall to CK level. To sum up, slow release-oxygen fertilizer significantly ease the decline of chlorophyll content and root vigor, restrain the MDA content, reduce plasma membrane relative permeability, improve the SOD、POD activity, reduce membrane lipid peroxidation and the damage caused by water stress, ensure the plant grow normally, which suggest apply slow-release oxygen fertilizer to improve the anti-water-logging of Macropanax rosthornii (Harms) C. Y. Wu ex Hoo.
Keywords/Search Tags:Flooded stress, slow-release oxygen fertilizer, bentonite, Macropanax rosthornii (Harms) C. Y. Wu ex Hoo, physiological and biochemical indexes
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