Studies On The Regeneration System Of Prunus Mume

Mei (Pruns mume), is a traditional ornamental plant in China.In recent years, studies on the regeneration system ofP.mume, as the premise for genetic transformation, is mostly concentrated upon the stem segments and embryo culture in vitro. The regeneration of adventitious buds through leaves is only reported in the’Meiren’, which is belonging to Meiren Group; and we do not have any report about successfully leaves regeneration in the other kinds of P. mume, which are majority breed of P. mume. Therefore, this study focuses on the establishment of P. mume regeneration system, by culturing the stem, leaves from P. mume’Sanlun Yudie’, P. mume’Xiao Lv’e’and P. mume ’Fenhong Zhusha’, which are respectively belong to Albo-plena Group, Green Calyx Group and Cinnabar Purple Group. The main conclusions are as follows:1. We compared the position, disinfection methods and culture conditions of the stem segment and leave explants. By culturing in dark, the growth activity of the stem segmentis better. Putting the leaves back up in the culture medium and then culturing in dark do good to induce callus.2. Through the study of the effects and interaction of TDZ,6-BA, IBA and NAA, the suitable leaf callus induction mediums for3kinds of P. mume are found out. For leaf callus induction, we take1/2MS as basal culture medium. Adding TDZ2.0mg/L,6-BA0.5mg/L, IBA0.5mg/L and0.1mg/L NAA, the leaves callus induction rate of’Sanlun Yudie’is85.04%. Adding TDZ1.0mg/L,6-BA0.5mg/L and IBA1.0mg/L, the leaves callus induction rate of ’Xiao Lv’e’ is79.76%. Adding TDZ1.0mg/L,6-BA0.2mg/L, IBA0.5mg/L and0.2mg/L NAA, the leaves callus induction rate of’Fenhong Zhusha’is79.66%.3. Through the study of plant hormones, which may have effect on the callus differentiation, the callus differentiation mediums for3kinds of P. mume are found out. Culturing in the1/2MS medium with TDZ1.0mg/L, IBA0.5mg/L and0.2mg/L NAA, the leaves callus differentiation rate of’Sanlun Yudie’is46.44%. Culturing in the1/2MS medium with6-BA1.5mg/L and0.2mg/L NAA, the leaves callus differentiation rate of ’Xiao Lv’e’ is34.21%. Culturing in the1/2MS medium with TDZ1.5mg/L, and0.3mg/L NAA, the leaves callus differentiation rate of’Fenhong Zhusha’is24.32%.4. By comprehensive evaluating the rate, the number and the length of rooting, we found out the suitable root differentiation mediums for3kinds of P. mume are found out. We also take1/2MS as basal culture medium. The rooting rate of’Sanlun Yudie’is88.29%and the rooting number is6.00, by culturing with6-BA0.5mg/L, IBA0.5mg/L and NAA0.3mg/L. The rooting rate of ’Xiao Lv’e’ is91.67%and the rooting number is6.03, by culturing with IBA0.7mg/L and NAA0.3mg/L. The rooting rate of’Fenhong Zhusha’is77.14%and the rooting number is4.12, by culturing with IBA0.3mg/L and NAA0.3mg/L.The results of this study show that the Leaf-explants Regeneration System of P. mume’Sanlun Yudie’, P. mume’Xiao Lv’e’and P. mume ’Fenhong Zhusha’ are successfully established for the first time. This work laid the foundation for the research of genetic transformation for P. mume through leaf disk, the research of plants resources protection and seed cultivation.