Studies On Dynamic Distribution Of IBDV-TJ-hg Strain In Main Organs Of Chicken

To observe the clinical symptoms and pathological features of the sick chicken of infectious bursaldisease in Tianjin. IBDV-TJ-hg strain (GenBank:JX846905) identified in the Lab was inoculated intochicken embryo for virus multiplication. Physical and chemical properties of multiplicated IBDV weredetermined and then animal regression experiment was carried out. Meanwhile clinical symptoms andpathogenesis were studied. The results showed that the chicken embryo virus was IBDV-TJ-hg, bursa andspleen were swelling postmortem. Histopathological observation showed that the inflammation of bursaand spleen were very typical. Those mean the pathogenicity of the strain is violent.In order to produce antibodies with high specificity, high affinity and high titers for immunohisto-chemistry experiments. In this study, IBDV-TJ-hg antigen was prepared from chicken embryo virusin Tianjin for health and adult rabbit immunity by multidrop hypodermic injection, and then purifiedantibody was analyzed. The antibody titer of Agar gel diffusion test was1:32, the protein contents were27.2mg/ml. The titer was unchanged and the protein contents were11.5mg/ml post-purification. Puritywas analyzed by U-V spectrograph analysis method and SDS-PAGE, purity of post-purificated antibodywas distinctly improved.To understand systematically pathogenesis of the occurrence, development and consequences of IBD,indirect immunohistochemical method was established and immunohistochemical condition for detectingIBDV was explored and screened. Distribution and infected period of infected chickens at different timesin different organs were studied by histopathology and immunohistochemistry method. The results showedthat IBDV-TJ-hg were only detected in cecum, liver, heart and lungs at1day after infection; IBDV-TJ-hgcan be detected in immune organs and non-immune organs at3day after infection, but the content ofIBDV-TJ-hg virus in fabricius bursa, spleen of these immune organs was far greater than in cecum, liver,lung and heart’s content of non-immune organs. IBDV-TJ-hg can not be detected in all non-immune organsat9day after infection, and IBDV-TJ-hg content of immune organs had reduced; IBDV-TJ-hg can still bedetected in immune organs at14day after infection; The duration of of fabricius bursa lasted until the21stday after infection.