Protein Feed With Monosodium Glutamate Wastewater And Cottonseed Meal In Solid-State Fermentation And Evaluation On Its Nutritive Value

Monosodium glutamate wastewater (MSG-wastewater) which is wastewater in the process of production of MSG, is a rich source of nonprotein nitrogen, if direct emissions not only caused environmental pollution, and waste of resources. Cottonseed meal (CM) is one of the excellent plant sources of protein, However, it contain some poisonous substances, such as free gossypol (FG) and anti-nutritional factors,which extremely restrict its uses in animal feed. Therefore, we will study of MSG-wastewater and CM by solid state fermentation, through the role of microorganisms to reduce FG in the substrate, and the nonprotein nitrogen into microbial protein, degrade the protein in conttonseed meal to proteins with small molecular, improve the quality of products of protein. In addition, study on metabolizable energy, energy utilization and amino acids utilization of protein feed by MSG-wastewater and CM during Solid-State Fermentation, CM and soybean meal(SM), with rooster egg and meat chickens for experimental on the animal.1. Study of screening strains on MSG-wastewater and CM by solid fermentationCM and MSG-wastewater and corn flour and bran powder according to the mass ratio7:1:1:1mixed as a substrate. First of all, solid-state fermentation by single bacterium, FG detoxification rate and protein changes of the fermentation product. The reduction effect of FG on the Bacillus licheniformis B12, FG from576.2μg/g to298.6μg/g, FG detoxification rate was55.12%; Bacillus subtilis Bs3had higher enzyme activity, respectively, at the same time water soluble protein and the content of small peptide was higher; Candida Candida utilis Cul total17kinds of amino acids was higher, at36.6%, the unleavened group significantly increased by6.3percentage points. On the basis of single bacteria fermentation, further to get the best fermentation strains. To free gossypol detoxification rate and free amino acids increased as indicators, ultimately determined the optimal strain which was Bacillus licheniformis B12and Candida utilis Cul.2. Optimized conditions of MSG-wastewater and CM by solid-solid fermentationTo Candida utilis Cul and Bacillus licheniformis B12as the starting strain, through the fermentation process to solid conditions and optimization of the medium, it was an optimization to a composite of solid state fermentation of high protein feed additive production process. First of all, single factor experiment of fermentation conditions for selecting inoculation amount, ratio of material to water, MSG-wastewate and inoculation ratio of four factors, lived bacterium of48h as evaluation index, to produce candida utilis Cul and bacillus licheniformis B12seed culture medium optimization. Optimum growth conditions of Cu1was pH6.0, MSG-wastewater by10%and glucose1.5%, temperature28℃; optimum growth conditions of B12was pH7.0, MSG-wastewater by10%and glucose1.5%, temperature of37℃.Secondly, added MSG-wastewater quantity, the ratio of material to water, inoculation amount and proportion of four factors, Inoculation ratio.The total amino acids as evaluation index, the optimum conditions of MSG-wastewater and solid state fermentation of CM for:MSG-wastewater by10%, the ratio of material to water while8, inoculation quantity was15%, Cu1and B12inoculation ratio of3:1.3. Evaluation on nutritional value with products by CM and MSG-wastewater in fermentationThrough research rooster egg and meat chickens respectively of the fermentation products, CM and soybean meal(SM) in the generation of energy and amino acid digestibility, fermentation products main nutrient utilization was discussed in this paper. We selected fermentation products and SM and CM apparent and true metabolizable energy and apparent and true amino acid utilization. Results showed that the fermentation products, CM and SM three apparent metabolic rate of the sample there were significant differences (P<0.05), in which SM the apparent metabolic rate of the highest, fermentation products. Fermentation products of true metabolic rate was significantly lower than SM of metabolic rate (P<0.05), slightly higher than the metabolic rate of CM, but no significant difference (P>0.05). Total utilization rate of the apparent and true amino acids in fermented products was the highest, respectively, significantly higher than that of CM (P<0.05), but no significant difference with SM(P>0.05).MSG-wastewater and cottonseed meal resources be full used, though microbial fermentation, free gossypol be reduced in the substrate, and the nonprotein nitrogen into microbial protein, improve the quality of products of protein. It has the high economic and social benefits.