Effect Of Auxin On Adventitious Root Formation Of Cephalanthus Occidentalis L.

Buttonbush (Cephalanthus occidentalis L.) is a kind of evergreen plant. It belongs to Cephalanthus of Rubiaceae in dicotyledons. Buttonbush has very good medicinal value, and its root, stem, leaves and flowers have been used in medicinal areas. Moreover, they can also be used as protective trees along banks and embankments. Agricultural university of Hebei bring in Buttonbush from Chinese academy of forestry and planted them in the nursery.In woody plants, adventitious root formation is a crucial step for in vitro clonal propagation. In many species, application of exogenous auxin is required to achieve rooting. As with other regeneration processes, adventitious root formation may be divided into three phases, namely dedifferentiation, induction and differentiation.According to the experiments, we found that:1. When IAA is used as the sole exogenous auxin, the optimal rooting culture medium for Buttonbush is1/2MS0+IAA10μM/L.2. When cultivate the cuttings (0.1cm) of Buttonbush in dishes, it is better to use terylene as the media between the cuttings and the medium.3. When cultivate the cuttings (0.1cm) of Buttonbush in dishes, in order to prevent the emergence of water vapour on the surface of the dish, the steps below are required:(1) Cultivate the cuttings in the biochemical incubator and insure the temperature in the inoculation room is not higher than that in the biochemical incubator.(2) Insure the temperature of the medium is not higher than that in the inoculation room.(3) Monitor the temperature changes of the experiment condition with the maximum thermometer and minimum thermometer.4. Endogenous auxin only used for the development of ARFP and it is not related to the initiation of ARFP.5. The speed of the development of ARFP is affected by exogenous auxin and related to its dose.6. Exogenous auxin could initiate additional-ARFP.