Cloing And Prompter Function Analysis Of Cotton Transcirption Factor GbMYBS

MYB transcription factor gene is one of the largest families in plants,which involved in responding to Abiotic Stress,regulation of secondary metabolism and the formation of plant organs.In this paper,based on the previous research of our lab,a new MYB transcription factor gene GbMYB5(GenbankNo.:JF820389) was isolated from Gossypium barbadenseby RT-PCR,The main results of this study are as follows:1、The expression pattern of GbMYB5in different organs was studied using RT-PCR.The results showed that GbMYB5was expressed in stems, leaves, buds, lints and seeds, whereas GbMYB5gene was highly detected in leaves.2、The expression of GbMYB5under abiotic stress was detected by RT-PCR.The expression of GbMYB5gene was inhibited in24h after heavy metals stress and recover to normal level in48h. Whereas the expression of GbMYB5gene increased significantly under PEG, ABA of GA treatment.3、A plant expression vector pCAMBIA2301-GbA/FBJwas successfully constructed, which driven by CaMV35S promoter and have a Kanamycin resistance selection marker.4、By heat shocking,we transformed the plasmid vectors pCAMBIA2301-GbMYB5into into Agrobacterium LBA4404successfully.The Agrobacterium strains containing pCAMBIA2301-GbMYB5infect tobacco leave,14kan-resistant plants were screened.5、The total RNA was extracted from the leaves of positive tobacco seedling,the RT-PCR showed the successful integration and expression of the transgene into tobacco genome.9plants expressed vector pCAMBIA2301-GbMYB5at RNA-level.6、A plant expression vector PBI101-T containing GUS was constructed for the further study of GbMYB5gene promoter. The vector was transformed into wild Arabidopsis by using agrobacterium-mediated method.GUS histochemical assay showed that GUS expression under GbMYB5promoter occured mainly in the roots and the seedling stages of transgenic Arabidopsis.