Study On Artificial Diet Of Loxostege Sticticalis L. Larvae And Its Effect Evaluation

Loxostege sticticalis L. is a Lepidoptera Pyralidae, also known as the green stripe pyralid, beet webworm; is a kind of worldwide distribution, conflagration pests.Its host plant species are more than35families200kinds.It seriously influences the normal development of northern crops in our country. Along with ceaseless development of Loxostege sticticalis L. investigation, researchers need a large number of physiological standard larvae, but larvae are restricted because that natural food could not be supplied all the year round for the larvae. Therefore, research of Loxostege sticticalis L. artificial diet solves the problem of seasonal shortage of food, and provides the health, uniform physiological development insect source for research and prevention trials, it is of great theory significance and economic benefits. The main conclusions are as follows:1.Through selecting empirically, we add leaf juice of Chenopodium album,leaf powder of Chenopodium album,leaf juice of alfalfa,leaf powder of alfalfa and the blank, reduce vitamin D, reed,cholesterol and so on in the diet, select empirically5kinds of formulations(HS、HG、MS、MG、CK), and the final result shows it is better to add Chenopodium album i.e. formula HG.2.Through orthogonal test, casein is a key factor of larval duration; casein, sugar and chenopodium album are key factors of the survival rate; casein, yeast powder, wheat bran and sucrose are key factors of pupation rate; casein is a key factor of pupa weight; paraben and inorganic salt are key factors of emergence rate. Therefore,these which are casein, yeast powder, wheat bran, sugar, nipagin, inorganic salt and chenopodium album are key factors of the overall development of Loxostege sticticalis L.. At the same time that the formula12is optimal in the16kinds of diet formulas of the orthogonal test.3.Through enzymes activity determination of midgut digestive,feeding effect of the formula HG is more close to Loxostege sticticalis L. natural food, but the quantity of sucrose is required to make a little change; the amylase, protease, esterase and lipase of the formula12are different from the control group,the quantity of protein,fat and sugar is required to do further revision and test. So the formula HG is better than the formula12.4.It makes the evaluation to the Loxostege sticticalis L. artificial diet selected out from the molecular level. By using the method of RT-PCR, carboxylesterase (CarE) gene mRNA abundance of the formula HG is higher than the formula12, slightly lower than the control group H, so the digestive ability of Loxostege sticticalis L. larvae to the formula HG is greater than the formula12, namely the formula HG is better than the formula12.