| This paper studied were collected from the four parts of the beef cattle samples analyze thedifferent ways to verify the effect of the analysis of local varieties of breeding and molecularexperiments, and for Chinese Simmental analysis, analysis of candidate gene polymorphismsassociated with carcass traits, and comprehensive analysis of the species through the discoverygenetic stability, to provide a reference for further improved varieties.This experiment were collected the growth and production traits, about138Xia nan cattle, Qinchuan136Simmental hybrid cattle176, and Chinese Simmental60,including height, weight,carcass weight, loin eye area. eye muscle weight, sirloin weight on brain weight, high-grade meatweight, marbling level and backfat thickness intramuscular fat,eye muscle area by B ultrasoundwhich by comparison of the data association analysis to discover the law of the growth ofproduction of four varieties., which provide a reference for the beef cattle breeding.In this study is divided into two parts, Firstly, compared the data processing to four species ofcattle, to identify the growth of production rules, and combined with t the actual situation toanalyze the experimental data and discuss the results; Secondly, part of the acquisition of60Chinese the Simmental sample for research material, the use of DNA sequencing, DNA sequenceanalysis by PCR-SSCP and other technologies, to detect the genetic variation of the MC3R,MC4R, Leptin analysis of its genetic structure and genetic diversity, and analysis the relationshipbetween the three genes polymorphisms and Chinese Simmental carcass traits, and moleculargenetics in order to explore the genetic effects of these genes in Chinese Simmental, find asignificant effect on the important economic traits genetic markers to provide a reference forChinese Simmental breeding improvement.It is noteworthy that, in the process of collecting the growth and production traits, theexperimental introduction of veterinary B ultrasound in vivo detection of production traits on thespecies of cattle, and collected after slaughtering the indicator correlation analysis found that thetime correlation coefficient of different species different varieties of genetically stable, one canconstruct the correlation coefficient, and thus more accurate and efficient service and breeding.Veterinary B ultrasound in vivo, the experimental measurement of eye muscle area, backfatthickness, measured after intramuscular fat and slaughter loin eye area, backfat thickness, intramuscular fat were analyzed by Chinese Simmental.The results were as follows:Part11. In the process of three varieties of the same trait found the weight of Simmental and Xia nancattle, Qin chuan the difference was significant (P <0.01), significantly higher than the other twovarieties; the Qin chuan backfat thickness compared to Xia nan cattle, Simmental difference wassignificant (P <0.01), significantly higher than those of Xia nan cattle and Simmental; the Qinchuan ocular weight was significantly higher than in summer Southern cattle and Simmental (P<0.05); the Qin chuan upscale meat production and Xia nan cattle and Simmental significantdifference (P <0.01), significantly lower than Xia nan cattle and Simmental.2. The comparation between the growth and production traits with gender in the Qin chuancattle,which found castrated bull of body height, weight, carcass weight, loin eye area, backfatthickness, sirloin meat weight, as well as on the brain meat weight,was very significant (P <0.01),bull’s body weight, loin eye area, brain weight, eye muscle weight as well as high-grade meatweight than cow of the data differences significant (P <0.05).3. Eye muscle area and backfat thickness of the four varieties of cattle were measured with Bultrasound, and found that the traits were slaughtered than the correlation coefficient differencesof the different species of conducting eye muscle area associated analysis found in the Simmental,the highest correlation coefficient (R=.656) the Qin chuan lowest correlation coefficient (R=0.148), found in the correlation analysis of the backfat thickness Xia nan cattle, the highestcorrelation coefficient (R=0.723).Chinese Simmental the lowest correlation coefficient (R=0.219).4. Between different species, eye muscle area and carcass weight, eye muscle weight, high-grademeat weight, as well as on the brain, and sirloin weight associated analysis found that, ChineseSimmental, loin eye area and carcass weight correlation coefficient of0.546; Xia nan cattlecarcass traits associated with eye muscle area coefficient for high-grade meat weight (R=0.756),the lowest correlation coefficient for brain weight (R=0.459); Qin chuan cattle in slaughter traitsassociated with eye muscle area coefficient highest Western cold weight (R=.658), the lowestcorrelation coefficient is on brain weight (R=0.374); Simmental slaughter traits and eye musclearea of the highest correlation coefficient for the Sirloin (R=0.820).the lowest brain weight (R=0.499).Part21. The varations of two loci in MC3R gene were studied. No polymorphism in MC3R genewas detected in the studied population. The varations of there loci in MC4R gene were studied. No polymorphism in MC4R gene was detected in the studied population.2. Relationship between genetic variation of Letpin gene and slaughter performance inChinese Simmental.The varations of four loci in Leptin gene were studied. Four SNPs (978T>C,3521G>C，3822C>T,4029T>C, ref. BTU50365) were detected in Leptin gene for thefirst time. The association between S978polymorphism and slaughter performance in ChineseSimmental was analyzed. The individuals carried978C allele had higher intermuscularfat.The association between S3521polymorphism and slaughter performance in ChineseSimmental was analyzed.The individuals carried3521C allele had higher intermuscular fat,carcass weight and marbling. The association between S3822and S4029polymorphismand slaughter performance in Chinese were analyzed.The results indicated that it didn，tinfluence the slaughter performance in Chinese Simmental. |
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