Marker-free Transgenic Rice Constructed By Co-transformation With Selection Marker OsPDCD5and The Target Genes

Rice is one of the most important foods of China and the world. The protection of rice production to ensure our food security and sustainable the agricultural development has important strategic significance. Discovery and cloning of rice genes of important functional traits and application of these genes through transgenic and molecular breeding approach can accelerate the process of rice breeding, and achieve new breakthroughs in rice breeding and production.OsPDCD5and LRK1gene were two genes cloned in the laboratory of Genetic and Plant Breeding of Fudan University.OsPDCD5is a programmed cell death gene linkaged into the expression vector pCAMIBIA1304(pC1304), and the Oshspl6.9promoter was instead of CaMV35S promoter, to obtained the expression vector of heat shock procedure death gene pC1304-Oshsp16.9-OsPDCD5. The expression vector and target gene were co-transformed into rice, and the recombinant progeny had selection marker OsPDCD5were dead under the heat hock treatment of42℃, as a result, it obtained the marker-free transgenic rice. When transformed the sequence of anti-OsPDCD5into rice, the salt tolerance increased. LRK1gene is the leucine rich-repeat receptor-like kinases gene. The trans-LRK1gene rice resulted in an increased number of branches, grains per panicle and grain weight, as well as LU Bai earlier. In this experiment, the gene expression vector pC1304-Oshsp16.9-OsPDCD5was used as a selectable marker, co-transferred into rice with the target genes. Ultimately,5individuals co-transformed of anti-OsPDCD5and8individuals transformed of LRK1were obtained. The transgenic plants were under heat shock of42℃to remove the marker.2individuals of marker-free trans-anti-OsPDCD5and3individuals of marker-free trans-LRK1were obtained. Experimental results showed that using the expression vector pC1304-Oshsp16.9-OsPDCD5can quickly remove the selectable marker. The transgenic plants can be used for further study on the function of anti-OsPDCD5and LRK1gene, and promoted the application of these2genes.