A Study On Transformation Of The Gene Encoding Human Adiponectin In Rice

Adiponectin (ADI) is a multimeric adipocyte-specific secretory protein from lipid metabolism and accounts for0.01%of plasma protein. Adiponectin is associated with insulin sensitivity with direct anti-diabetic,anti-atherogenic and anti-inflammatory properties, and also reported to possibly function as an factor which related to the insulin sensitivity. The levels of adiponectin in patients of type2diabetes and cardiovascular disease is generally lower than normal. In this study, we introduced the modified ADI gene into rice via Agrobacterium, which may give some foundation to build rice as plant bioreactor to product more cheep ADI.Two japonica rice cultivars, Wuxiangjing9and Wuyujing3,were used as the receptors, and were transferred with the vectors containing synthesized and optimized ADI gene by using Agrobacterium-mediated transformation. We got a large number of transgenic rice plants. After several generations, we got the homozygous transgenic rice lines in T6generation from Wuxiangjing9, and some in T2generation from Wuyujing3. Then we extracted the DNA, RNA and protein from homozygous transgenic lines and performed for RT-PCR, Northern blotting and Western blotting analyses, respectively. The results showed that the introduced ADI gene successfully integrated into the rice genome. Analysis of expression showed that the foreign genes could transcript in majority of transgenic plants, and can accumulate proteins in the mature seeds of transgenic plants. The study lays a foundation for further study of ADI from rice.