Molecular Epidemiology Detection And Characterization Of Porcin Group C Rotaviruses In Lulong, Hebei Province Of China

Objective To study the infection rate of group C rotavirus and the diversity of VP6gene, andthen to understand the molecular epdimiology characteristics of other major gene segments（VP4and VP7）in Lulong area, at last to provide basis for understanding the relationshipbtween porcine, human and other GCRVs，and provide basis for preventing swine fromGCRV infection.Methods Collect793stool specimens from Lulong porcine with diarrhea or not within thewinter of2007and the spring of2008. GCRVs were detected by nested multiple reversetranscription-polymerase chain reaction (nested RT-PCR) using the specific primers, and thenamplified and cloned the VP7and the partial VP4fragments of6positive specimens whichwere determined with random choice method. The sequencing results were analysed at last.Results GCRV was detected in132out of793(16.65%) speciments from Lulong. Analysisresults showed a large-span homology. Homology and phylogenetic analysis indicated thatporcine GCRVs were with significant diversity and widespred. It was South Korean GCRVswhich were the most related to the gene VP6of Lulong GCRVs. PCR and cloning werecarried out for the VP7and VP4genes of6positive GCRV strains. Sequence analysis forgene VP7indecated that Z167, Z761and Z663were near to G1genotype strains, while Z638to G3and Z776to G6. But Z677was far from any G genotypes. Only the VP7fragment ofZ776has a highest homology among all VP4and VP7gene fragments of Lulong porcineGCRVs.Conclusion The infection rate of GCRVs was high from the winter of2007to the spring of2008in Lulong area，and the gene fragments had large difference in molecular. Theprevalence of Lulong porcine GCRV was similar with this of South Korean，and there might be genetic ressortment.6Lulong positive GCRV strains shared G1, G3and G6genotypes,respectively, meanwhile, G genotype couldn’t be dertermined for one strain Z677. Theexperimental data provided a basis for further study of molecular epidemiology of the porcineGCRVs and the relationship between porcine and human GCRVs.