| Baculovirus possesses a double-stranded DNA genome ranging from80kb to180kb. Baculovirus only infects the arthropods, including the lepidoptera, hymenoptera, and diptera. During its life cycle, baculovirus produces two types of viral virion:the budded virus (B V), which is responsible for the spread of virus from cell to cell within an insect, and the occlusion-derived virus (ODV), which is responsible for the infection between insects.AcMNPV (Autographa californica multiple nucleopolyhedrovirus) has been extensively studied as a model virus.It is well documented that baculovirus infection and actin skeleton of host cells have close relationship. After baculovirus entering insect cells, it immediately induces actin polymerization, which helps the nucleocapsid penetrate into the nucleus. At late infection phase, baculovirus induces nuclear actin polymerization, which facilitates the nucleocapsid assembly. The baculovirus-induced actin polymerization is mainly associated with the virus nucleocapsid protein P78/83, which is homologous with N-WASP protein. The mechanism of how the baculovirus and host cells regulate P78/83, and Arp2/3complex nuclear entry remain unknown.Our research mainly includes two aspects:(1) The stability regulation mechanism of P78/83. In this research, we identify the C42-P78/83interaction domain. We also find that the insect cells can degrade the P78/83protein via its N terminal amino acid sequence. However, C42can stabilize P78/83by interacting with P78/83.(2) Cloning and functional analysis of the Arp2/3-ARPC40subunit from Sf9cell line. We cloned the full-length cDNA sequence of ARPC40by RACE. We provide evidence that ARPC40can interact with P78/83only in virus-infected cells.We also analyze the subcellular location of ARPC40. In uninfected cells, ARPC40mainly locates in the cytoplasm, which is in contrast to its nuclear localization pattern in virus-infected cells. |
