| In this study, leaves harvested from10year-old mother plants of loquat (Eriobotrya japonica L.) were used as explants for in vitro culture. The objectives were to elucidate the effects of different pretreatment, different basic media and different plant growth regulators on the callus induction, proliferation, shoot differentiation and rooting, also the effects of different browning inhibitors on browning control were studied. The full design, orthogonal design, combined with analysis of variance, multiple range annalysis, multiple comparisons and other statistic methods were employed to determine the factors and culture conditions. The results were as follows.1. The moderately mature leaves around10days after leaf expansion were optimum for callus induction at a high rate of89.17%。2. Before culture, the explants were treated with75%alcohol for20s, then0.1%HgCl2for10min, then the best sterilization effect can be achieved at a lowest contamination rate of13.33%.3.0.5mg/L AgNO3showed the best effect on browning control at a rate of23.33%; AC showed the worst effect with a high rate nearly100%.4. The optimal induction of calluses was achieved on MS medium supplemented with6-BA0.5mg/l and2,4-D0.5mg/l, at a high frequency of89.2%.5. The most suitable medium for callus proliferation was MS medium supplemented with6-BA0.5mg/L and2,4-D0.2mg/L. TDZ0.8mg/l, NAA0.3mg/l and AgNO30.2mg/l were appropriate for callus induction, in which the induced calluses were green, dense, granular.6. The most suitable medium for shoot differentiation was MS medium supplemented with TDZ0.8mg/l, NAA0.3mg/l and AgNO30.2mg/l, in which shoots were regenerated at a rate of3.89%. 7. Complete plantlets were succesfully obtained on MS medium supplemented with the same components of the best shoot induction medium plus0.2mg/l IB A at the rate of83.33%. The rooted plantlets, after hardening, survived at a rate of90.00%in the transplantation matrix of autoclaved sawdust, peat and humus (1:3:1) under careful temperature and water management. |
