Bovine Brucellosis Serological Investigation In A Region Of North China And Formation Of Multi-PCR Detecting For Brucellosis

To understand the situation of brucellosis in a region of north China and apply the more effective precautions, this study applied epidemiological survey stochastically in the grazing farms and distributive cattle groups in this region, using Rose Bengal Precipitation Test(RBT),Serum Agglutination Test(SAT) and Indirect Enzyme Linked Immunosorbent Assay(I-ELISA), and compared the three experiments, respectively. Based on the brucellosis gene sequence published on GenBank, specialized primer was synthesized, multi-PCR identification and diagnosis of Brucella and brucellosis on cows and sheep were established and primary application test was already conducted.Stochastically using the three methods above,950serum samples from the grazing farms and distributive cattle groups in a region of north China were inspected. Results showed that the epidemic situation of brucellosis is relatively serious. However, positive rates greatly vary among groups with the highest50%and the lowest4.6%in the grazing farms and no brucellosis were found in the breeding bull farms and the positive rate in distributive cattle groups was39.3%; RBT tested105serum samples and the positive rate is15.8%; SAT tested128serum samples and the positive rate was19.2%; I-ELISA tested262serum samples and the positive rate was27.6%. Results show the I-ELISA enjoys the greatest positive rate, higher than SAT and RBT. RBT ranks as the last.The sensitivity of multi-PCR could reach approximately100pg, possessing excellent specificity and repeatability. PCR amplification of7staphylococcus samples appeared negative. Primary clinical sample applications indicate this method could be used for dairy samples, vagina and excrement cotton swab test.