| In recent years, a kind of special Ca2+sensor: calcineurin B-like pro-teins（CBLs）were identified in plant. Interaction with CBL-interacting protein kinase（CIPK）, they play an important role in the process of the growth of the specific de-velopment and response to stress in plant. In the model plant arabidopisis thaliana andthe rice, the study found that CIPK widely participate in high salt low potassium,drought and abiotic stress response. The current research of CIPK focus on the arabi-dopsis and rice, the research are relatively few on other plants. Corn as an importantfood crop in the world, drought, poor soil and abiotic stress is the important factorswhich limit its production. Excavatation of CIPK gene function analysis, not only hasthe important theoretical significance, but also the value of the extensive application.The effective candidate gene was provided for the potential breeding of geneticallymodified corn. Earlier research in our laboratory based on the work, five ZmCIPKsgenes were screened and do further researches, and the main research results are asfollows:1, In drought stress, the expression of ZmCIPK1, gradually raised in leaf, In rootsincrease at first then drop; In4℃the expression is changed little; The expression ofZmCIPK3, gradually raised in leaf, but almost no expression in root, after4℃stress,there is some expression in the root, and no expression in root; In normal conditions,there is a certain amount of basic expression of ZmCIPK8, in leaf and root, and in theleaf change significantly. By salt, cold and PEG induction, there is some expression ofZmCIPK8, but not under the ABA condition; The of expression of ZmCIPK17is sim-ilar with ZmCIPK1in drought, a large increment of expression after4℃stress;ZmCIPK18mainly affected by drought stress, both of the leaf and root, the mainlyexpression is in the root after4℃treatment. Further comparison, ZmCIPK8was sig-nificant affected by drought stress. 2, Cloning and sequence analysis of ZmCIPK8gene, to gain ZmCIPK8cDNAfull length by the method of PCR, analysis ZmCIPK8genome using online tools（http://www.plantgdb.org/）, contains14extrons and14introns, the protein structureof ZmCIPK8has the N terminal kinase domain and the C-terminal NAF structuredomain that is shared by all the CIPKs.3, Simultaneously, using PCR got the promoter sequence of ZmCIPK8, analysisof the components forecasting of ZmCIPK8promoter, this gene’s promoter region haslight response, stress response, development related, hormone related and other un-known functional domain. Currently, discuss the function of ZmCIPK8promoter bythe report gene of GUS though the genetically modified method.4. Construction of plant expression vector of pBI121-ZmCIPK8using ofZmCIPK8gene has been cloned, transform the tobacco though Agrobactrium tumefa-ciens, identification of the positive strain by PCR amplification and the screen of re-sistance, tested the transcription expression of ZmCIPK8from the mRNA level. Thedetermination of the stress tolerance related physiological and biochemical indexeschlorophyll, superoxide dismutase, malondialdehyde and proline, results showed thattransgenosis strain of ZmCIPK8is obviously superior to the wild type tobacco aboutthe resistance. Further to the analysis of the expression of the stress related transcrip-tion factors NAC, RD29A, CBF and so on, the results showed that the expression ofthe genetically modified strains are obviously higher than those in the control. |
