Research On Population Structure And Genetic Diversity Of96Maize Inbred Lines

Maize (Zea mays L.) is one of the most important cereals and forage crops in China, and plays a key role in Chinese agriculture. Genetic diversity and heterotic grouping among maize germplasm are fundamentally important in mazie breeding. To define heterosis group and heterotic patterns, it is tremendously for improving the efficiency in maize breeding. The96maize inbred lines in this paper were from College of Agronomy of Xinjiang Agricultural University. Target groups and fingerprinted with108simple sequence repeats (SSRs) covered the entire maize genome, using the STRUCTURE software for population genetic structure assessment. And analysis the genetic diversity among96maize inbred lines. Advantages for the preparation of hybrids provide a theoretical basis. The results were as follows:(1) Fingerprinted with108simple sequence repeats (SSRs) covered the entire maize genome, a total of472alleles was detected across all the96maize inbred lines, ranged from2-9. The average genetic diversity was0.6143, and the minimum and maximum of genetic diversity were0.1838-0.8475. The average polymorphism information content (P1C) was0.5535, and the minimum and maximum of polymorphism information content (P1C) were0.1674-0.8019. All the parameters indicated a rather high level of genetic diversity of the maize inbred lines.(2) Using the STRUCTURE software to evalue the population genetic structure assessment. Setting subsets (K) for1-9, using ΔK (k) to determine the most suitable the value of K, and then generates the Q-matrix. Assessment of population structure, that the most appropriate K=2and the cluster analysis showed that the inbred lines could be classified into2distinct clusters.(3) A distance based clustering method and a model based clustering that they had same results of the whole set of96maize inbred lines.96maize inbred lines were grouped into three cluster by the distance based clustering method. The results of the sweet corn self-contained group, are consistent with the model classification.