Study On The Genetic Transformation Of Sea Island Cotton And NAC6Transcripts Family Gene

In this study,to induct different genotypes fo sea island cotton hypocotyls to somatic embryogenesis,using the embryogenesis callus of Xinhai16, Xinhai24and Xinhai30as acceptor materials, then further optimizing the genetic transformation system of sea island cotton, transforming NAC transcription factor family through agrobacterium-mediatde method to get transgenic regeneration plant To, and then taking osmotic stress on cotton somatic embryogenesis with PEG6000(referred to PEG),by studying the sensitivity of somatic embryos to different concentrations of PEG stress, the research contents and the results are as follows:Using the established cotton regeneration system, compare regeneration plants and the regenerative plants of island cotton and land cotton in induced callus period, further define the regeneration plants have advantage over regeneration plants in somatic cells happen. The results show that the regeneration plants reduced half time compared with regeneration plants in various periods.Agricultural Biotechnology Xinjiang Agricultural University,Urumqi830052;China) Abstract:In order to further optimize agrobacterium-mediated the exogenous gene of sea island cotton transformation system, improve conversion rates. With the embryogenesis callus of Xinhai16, Xinhai24and Xinhai30as explants, through the callus cultivating to get tested embryo callus and use them as receptor materials, and use the established exogenous gene transformation system of island cotton as the basis, and use the variance analysis to study the conversion effect of various strains, different plasmids agrobacterium-mediated island of cotton embryo callus genetic in the process of transformation. The results show that, strains have certain effect on conversion, while the plasmid significant. Eventually get the best agrobacterium-mediated and plasmid sea island cotton embryos callus species are:GV3101and pBI121.This study result will be contribute to improve the Genetic Transformation System of the sea island cotton callus cultivating.Stress embryogenic callus by the PEG6000of different concentrations to study the induction rate, and get the changes of the transgenic and non-transgenic island cotton obtained drought tolerance level by measuring the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), malondialdehyde (MDA), proline (PRO). The results show that:the drought tolerance level of the transformation Xh30gene of NAC6is the best when the concentrations of PEG6000reaches10%,with the concentrations raised of PEGthe activity of island cotton gradually cut down and die eventually.