Isolation And Purification Of Antifungi Active Compound And Biological Activity From Conyza Canadensis

1.Ethyl acetate and butanol extractions from Conyza Canadensis Aqueous extracts were used to test their allelopathic effects on the seed germination and seedling growth of three weeds (Echinochloa crusgalli (L.) Beauv, Bidens pilosa L, and Mimosa pudica L). The results showed that ethyl acetate extract could strongly inhibited the seed germination and seedling growth of E. crusgalli (L.) Beauv and B.pilosa L. The inhibition increased with the increasing concentration of extraction. High concentration of the Aqueous extract could effectively inhibit the seed germination and seedling growth of M.pudica L. When the concentration of ethyl acetate reached0.5mg/mL, significant differences of three seedlings emerged on their root length, stem length and fresh weight compared with the control. When the concentration of ethyl acetate reached2mg/mL, the RI of the root length of E.crusgalli (L.) Beauv and B.pilosa L was both-1while the RI of the stem length was-0.97and-0.78respectively. The average germination rate of two seeds was3.3%and2.2%respectively. The butanol extraction could inhibit the seed germination and seedling growth of three weeds, but its inhibition is more weak than the ethyl acetate extracts. High concentration of the Aqueous extract could effectively promote seedling growth of three weeds. It could be concluded that herbicidal active substances were probably existed in extracts of ethyl acetate from Conyza Canadensis.2. Using growth rate method to test ethyl acetate and butanol extractions from conyza Canadensis aqueous extract gloeosporium musarum, colletotrichum gloeosporioides, and botryodiplodia theobromae the inhibition of three types of plant pathogenic fungi and tested the EC5o of the three fungi by ethyl acetate extract. Results showed:ethyl acetate and butanol extractions has inhibition effect towards the three kinds of fungi. Under condition that ethyl acetate extraction setting at a minimum of0.5mg/ml, the average pathogen circle diameter of three kinds of fungi had a significant difference comparing with reference. Along with the ethyl acetate extraction concentration increased or decreased, reaching a peak low point under2mg/ml, bringing the current inhibition rate to an average of90%and higher. When Butanol extraction was at high concentration the average pathogen circle diameter of three kinds of fungi and lowest setting concentration of0.5mg/ml has no significant difference. EC50of ethyl acetate extraction to Gmusarum, C. gloeosporioides and (botryodiplodia theobromae) was0.804,0.917and 0.643mg/ml.3. By using methods of spore sprouting on slides and detached leaves determined biological activities that C.canadensis ethyl acetate extraction made effects on C.gloeosporioides and Gmusarum cooket mass, also determined the change of enzyme activities after extraction prevented C.gloeosporioides. Results showed:C.canadensis ethyl acetate extraction had a strong inhibition effect towards two kinds of pathogens, with the increase of extraction concentration inhibition ability increased, when setting highest concentration2mg/ml reached its peak, inhibitory rate to C.gloeosporioides and Gmusarum cooket mass was87.70%and94.27%. High concentrations of C.canadensis extract to Gmusarum cooket mass on leaves of Musa paradisiaca had low levels of inhibition, had no significant trend to C.gloeosporioides on the leaves of Hevea brasiliensis.4.After using C.canadensis ethyl acetate extraction to dispose seeding of H.brasiliensis which was infected by C.gloeosporioides, determine the change of physiological index inside leaves of seeding of H.brasiliensis. Results showed:SOD and POD enzymes in leaves of seeding of H.brasiliensis increased at first and then tended to smooth with the increasing of concentration.MDA decreased at first and then increased with the increase of concentration, the content of MDA in leaves of H.brasiliensis reached a peak low point under8mg/mL.37.02%lower than reference. Disposing seeding of H.brasiliensis with extractions of high concentration, enzyme activity of PAL in leaves was higher than reference, reached its peak under8mg/mL,30.98%higher than reference.5.Through using methods of active tracking and Silica gel column chromatography to separate and purify antibacterial substances of C.canadensis, anaylize the structure of Active ingredients by using modern spectroscopic, determine the effect that active ingredients took on Pestalotia palmarum,Colletotrichum gloeosporioides and Fusarium oxysporum three kinds of pathogenic fungi by using Filter Paper Method. Results showed:Antibacterial active compounds XFP3.7of C.canadensis which was separated from Fr.3-7by using active tracking was identified to be4-Ethyl-1,2-benzenedioI(CAS:1124-39-6)and was found Inhibitory effect on three kinds of Pathogenic fungi in various degrees.