Effect Of Different Levels Vitamin A,Vitamin E In Broodstoek Diet On Reproductive Pertormance Of Turbot (Scophthalnws Marinws)

Feeding trials were conducted to investigate the effect of dietary vitamin A and vitamin E on the growth and reproductive performance of turbot(Scophthalmus maximus). During the trials, water temperature ranged from8to14℃, salinity from30to31‰, pH from7.2to7.5and dissolved oxygen content was approximately6mg/L. The main contents are the following:1. The experiment was conducted to investigate the effects of vitamin A with different supplementation levels (0,10000IU/kg,80000IU/kg diet) on growth and reproductive performance of turbot(Scophthalmus maximus). Ninety30-month-old turbot weighing1415.8±23.4g were randomly assigned to three groups, and cultured trials lasted eight months. Average relative fecundity, percentage of buoyant eggs, fertilization rate, hatching rate of eggs, size of eggs and length of fry, were used to evaluate the performance of the turbot fed with different diets. The results showed that percentage of buoyant eggs and hatchability of eggs of the group which were fed with80000IU/kg vitamin A was significantly higher than those of control group (P<0.05). The body length of fry of the group which were fed with80000IU/kg vitamin A was2684.1±24.91μm, which was significantly higher than those of control group2562.3±30.00μm (P<0.05).The content of n-3HUFA, n-6HUFA in eggs, the vitamin A in gonad increased with the increase of the diet vitamin A supplementation levels. SOD activities in serum of broodstock which were fed with80000IU/kg vitamin A was91.88U/mL, which was significantly higher than those of control group70.57U/mL (P<0.05)2. The experiment was conducted to investigate the effects of vitamin E with different supplementation levels (0,200mg/kg,700mg/kg diet) on growth and reproductive performance of turbot{Scophthalmus maximus). Ninety3-year-old turbot weighing1478.6±16.9g were randomly assigned to three groups for five months. To study the effect of dietary vitamin E on the growth of turbot broodstock and the quality of sperm, we compared the difference of growth, sperm volume, the sperm lifespan, the sperm concentration, the sperm morphology, testis-somatic index (TSI) and so on during three experimental groups. The results indicated that there was no significant difference in growth between E700group and control group (P>0.05). TSI in E700group was significantly higher than that in control group (P<0.05).No significant differences in activation rate and eddy time of sperm were found between E700group and control group (P>0.05). Difference in sperm volume, sperm lifespan, sperm concentration and number of mitochondria in sperm between E700group and control group was significant (P<0.05), but there were no marked difference in those between E700group and E200group (P>0.05). The content of vitamin E in gonad and liver in E700group were significantly higher than that in E200group (P<0.05), and the content of that in control group were significantly lower than it in E200group (P<005).The quantity of DHA, EPA and n-3HUFA in sperm increased following the increase of dietary vitamin E.