Screening Of Microsatellite Molecular Marker And Genetic Diversity Analysis For Mud Carp

Mud carp (Cirrhinus molitorella) belongs to Cypriniformes, Cyprinidae,Labeoninae family, Cirrhinus,distributing in Pearl River, Ming River, Han River,Hainan Taiwan and southern of China, abroad southeast of Asian. It is one of the fourmain fish species for cultivation and fishing in Guangdong province. Due to thefeeding wide source, strong disease resistance, groups of high output and able to adaptto a more fertile water body, it’s become major aquaculture species of southern China.At present with the worsening environmental pollution of the water body and thedifferent degrees of human subjective intervention control on mud carp groups, led tothe inevitable result was its genetic diversity decreased. Resulting the decreasing ofability to withstand environmental change, slow growing and other phenomena. Usingmolecular genetic markers on the mud carp genetic diversity analysis and to seeksuitable protective measures, to achieve the purpose that to protect the germplasmresources and to keep the sustainable development and utilization is very necessary.In this study, each31individuals DNA templates of big and small groups wasused to isolated microsatellite DNA markers from the two experiments respectively,the same DNA templates were from wild light color mud carp, Cirrhinus molitorellafrom Xi Jiang population. One of the experiments was the contrast experimentbetween the developing of microsatellite molecular marker and the screening ofmicrosatellite molecular marker, and the other was the genetic diversity analysis ofmud carps. Aims to gain the highly applicable and specific of microsatellite DNAmarkers for mud carp, which could be used with the genetic diversity analysis to themud carp and other fishes. The experimental conclusion could be used in the researchof the genetic diversity research, population genetic structure analysis, populationgenetic relationship identification and protection of germplasm resources etc.1The comparative experiment between the development and screening ofmicrosatellite DNA markers in differental groups According the magnetic bead hybridization method, it was obtained23parismicrosatellite primers, the15pairs can be stable amplification and the primer gainedefficiency was65.22%,14pairs were polymorphic primers, the proportion was60.87%; screening the close relate fish primers found33pairs microsatellite primers,the10pairs can be stable amplification and the primer gained efficiency was30.30%,polymorphic primers was8pairs, the proportion was24.24%, the average PIC were0.5933and0.5692respectively, the former was as well as higher than the latter.Although the high abundantly polymorphisms of both the groups, but the former was2.15times than the latter, that magnetic bead enrichment method was better than theclosely related fish primers screening method for obtain the high efficiency andavailability of microsatellite primers for mud carp.2Genetic diversity analysis in mud crapIn this paper,25pairs stabilized and amplification microsatellite primers wereacquired from the two methods, there were3single polymorphic loci primers,7pairsmoderate polymorphism loci primers,15pairs highly polymorphic loci primers; therange of PIC of all the loci were from0.2885to0.8457and the average PIC was0.5846. The range of PIC on the small and big groups were0.2885～0.8050and0.2885～0.8256respectively, the average PIC were0.5615and0.5846respectively.The appearance frequency of locus b of highly polymorphic primers4were61.3%,22.6%respectively in big and small group, the fragment length of the locus was119bp. The frequency in the big group was higher nearly3times than in the small, thislocus may be candidate differential molecular markers.