Evaluation Of Releasing Studies In Chinese Shrimp (Fenneropenaeus Chinensis) Using Molecular Marker

Chinese shrimp (Fenneropenaeus chinensis), as an annual large economic shrimpand one of the important fisheries resource, chiefly distributed in the Yellow Sea and theBohai Sea.To the1980s, its resources have been depleted as the influence of overfishing,water pollution and engineering construction. Fishery resources enhancement is aneffective method on the ecological environment of waters repair and fishery resourcesresume. The production of enhancement and the releasing of transplant of Chineseshrimp were developed at the early1980s in China, the economic benefits and socialbenefits were gained as this work is taken. However, a kind of method about theevaluation of resources enhancement in Chinese shrimp has being lacked by the reasonof releasing marker. The molecular marker is used for discuss the new evaluationmethod of resources enhancement effect, as the aim of providescientificbasis in theenhancement and the releasing of Chinese shrimp. The results as follows:Microsatellite marker is a kind of molecular marker that codominance in genetics,Mendel genetic law followed. So five loci, FCKR002, FCKR005, FCKR007, FCKR009and FCKR013, rich in polymorphism and selected from the microsatellite sequences ofindependently developed Chinese shrimp, combine the three existing microsatellite lociof high polymorphism EN0033, RS0622and RS1101, to form two groups of quadruplePCR reaction systems with the primers inside the groups marked with fluorescencelabeling of6-FAM, VIC, NED and PET. Two groups of microsatellite quadruple PCRreaction systems are established through optimization, and these2reaction systems canbe used preferable in genetic subtypes.The examination on genetic diversity shows that225alleles were found in eightloci of200individuals, with the average loci per allele as28.13and the average PIC value as0.8939. Two hundred individuals were randomly selected from the populationin the releasing simulation evaluation experiment, and then individual/pedigree tracinganalyses were conducted on these200individuals with the two micro-satellitequadruple PCR reaction systems for fluorescence labeling. The result of shows that ifparents have already been known, the offspring of three full-sibs families could beappropriately identified by these two groups of quadruple PCR systems.The low temperature reaction system group was used for genetic subtypes inreleasing simulation of1786individuals.A kind of computer program, Cervus, showsthe result that the molecular identification is191/300, as the family result is35/100,64/100and92/100, the VIE marking identification is186/300, as the family result is34/100,60/100and92/100.The detection ratio of molecular marker is greater than theVIE marking, so the high temperature group reaction system was used to enhance theexclude ratio, and the result shows that molecular marker has more advantage than theVIE ones.Moreover, the genetic structure between individuals in releasing simulation andmixed background group was analyzed and we study the proportion in the number of“internal standard” which to be thrown in. The result shows that no matter the markingindividuals are existing or not, the He value shows no significance (p>0.05). During theenhancement and the releasing,“internal standard” is an important part, so for the aimof accurate evaluation in the effect of releasing, we should chose the same “internalstandard” as close as possible.