The Impact Of Photoperiod And Temperature On The Sex Differentiation Of Cucumber

Cucumber (Cucumis sativus L.) belongs to Cucurbitaceae crops, is cultivated widely in countries around the world a kind of vegetables and fruit vegetables, an important production and economic status. Performance because of their gender diversity, physiology and genetics of a wide range, cucumber has become a model plant for research on sex differentiation. Sex like other trait, control by the genetic material, but sometimes the en vironment can effect or even change the sex, therefore has a close relationship of environment and sex differentiation. Sex differentiation manifested at the molecular level for sex differentiation process involves the induction of genes occur signaling role to derepression, and by the time and spatial sequence expression of a specific product. Environment by effect the expression of genes effecting plant growth and development and gene expression products.In this study, flower bud differentiation was obserbated by physical dissection microscope to explore the temperature and photoperiod on the impact of sex differentiation of cucumber. By the results, Solexa sequencing on the Cucumber "C09-123" which is Cultured in the high night temperature and low night temperature conditions.Main results were as follows:1) Temperature and photoperiod on sex differentiation of cucumber had an impact, and temperature on the sexual differentiation of cucumber than photoperiod.2) The12℃produced the most female flowers, and the24℃produced no female flowers.3) The genome of Cucumber was sequenced by Solexa approach. And the Cucumber "C09-123" genes were studied by comparative genomics. We detected15781genes differentially expressed between Cucumber "C09-123" which is Cultured in the high night temperature and low night temperature conditions. And a total of1654remaining genes were considered expressed, among them507were down-regulated and1147were up-regulated according to the analysis (FDR <0.001and≥1-fold). Gene ontology (GO) analysis was performed. GO analysis revealed that in molecular function63%genes identified as binding, such as protein binding、necleotide binding and calcium binding, other genes like hydrolase activity、transferase activity、kinase activity is highly correlated. In biological process, cellular nitrogen compound metabolic process’, gene expression、cellular component organization、cellular biosynthetic process、response to organic substance is highly correlated. In cellular component, plastid stroma、protein complex、cytoplasmic vesicle is highly correlated. There were108signal pathways were related and9of them revealing closely connected to low night temperature affect cucumber C09-123"sex differentiation were citrate cycle、galactose metabolism、fatty acid biosynthesis、ubiquinone and other terpenoid-quinone biosynthsis、photosynthesis-antenna proteins、porphyrin and chlorophyll metabolism、diterpenoid biosynthesis、phosphatidylinositol signaling system、RNA transport.