Influence Of Cells Apoptosis Of Probiotics Chicka Vaccinated With Ibd Vaccine

One day old chicks fed with probiotics and vaccinated by IBD vaccine and then was attacked by virulent IBDV were used in this study. RT-PCR,ELISA,HE,TUNEL and other technical were used for revealing the changes of apoptosis related gene bcl-2mRNA,caspase-3,9activity, the number of the apoptosis cells in immune organs-thymus, spleen, bursa of Fabricius, as well as the dynamic changes of nuclear cytoplasm ratio in these immune organs. The study aimed at revealing the influence of apoptosis when chicks fed with different probiotic combinations and then vaccinated by IBD vaccine comprehensively, which can provide a scientific basis on probiotic research and development. The results showed that:1. Chicks fed with probiotics, bcl-2mRNA of spleen, bursa of Fabricius were significantly higher than control chicks in4-7d; caspase-3,9activity was much lower than control chicks in7-11d; compared with the control chicks, there are no significant changes of the number of apoptotic cells and the nucleus cytoplasm ratio of thymus, spleen, bursa of Fabricius.2. Combine with IBD vaccine, chicks fed with probiotics,which the bcl-2mRNA expression of the immune organs much higher than the control chicks and the chicks vaccinated by IBD vaccine alone. caspase-3,9enzyme activity were higher than the control chicks, but lower than the IBD-vaccinated chicks. After IBD vaccinated, the apoptosis cells of the chicks fed with probiotic lower than the chicks without probiotic, the immune organs apoptosis apparently, which nucleus cytoplasm ratio reduced correspondingly. However, compared with chicks untreated by probiotic and IBD vaccine, the apoptotiic cells of immune organs of IBD-vaccinated chicks were more.3. After the challenge of virulent IBDV, bcl-2mRNA expression in cells of immune organs-thymus, spleen and bursa of Fabricius in chicks fed with probiotic were vaccinated by IBD vaccine was much higher than the chicks vaccinated by IBD vaccine alone. Caspase-3.9activity and the number of apoptotic cells were much lower than the chicks vaccinated by IBD vaccine alone, but the nucleus cytoplasm ratio of immune organs was higher than the chicks vaccinated by IBD vaccine alone. The study reveal that:the probiotics can effectively regulated the expression and activation of anti-apoptotic factors in chicks, to resist the immune organs apoptosis when they face challenge of virus, to keep the immune orangs of chicks normal growth and development, and to improve chicks’immunity.4. Whether only fed chicks with probiotics or probiotic-fed chicks vaccinated by IBD vaccine, the promotion of ability of anti-apoptotic in chicks, probiotic based on Lactic acid bacteria were much better than the probiotic based on Bacillus.