Study Of Diversity Of Eukarytic Plankton In The Sea Area For Scallop Culture

The scallop, Chlamys farreri, is used to be one of the marjor species cultured inNorth China. However, the great expansion and in tensification have induced theoccurrence of disease since1997which cause apparent stagnation to the developmentof the scallop industry. The disease has been becoming epizootics in Shandong andLiaoning provinces from then on. Epidemiological and pathological study shows thatthe pathogen of massive death of the scallop was acute viral necrobiotic virus(AVNV).And that the scallop could be infected by taking the plankton carried AVNV as food,and the plankton was infector of AVNV were proved. In order to study the diversity ofeukarytic plankton carrying AVNV in the sea area for scallop culture, this study applyDGGE, Clones libraries and PCR-RFLP technologies to analyze Diversity ofEukarytic plankton and its seasonal changes.In order to study the diversity of eukarytic nanoplankton in the sea area for scallopculture, and clarify the relationship between eukaryotic nanoplankton andtransmission of zhikong scallop (Chlamys farreri) acute viral necrosis virus (AVNV),seawater samples were collected monthly in2009and2010from Qingdao LiuqingRiver Bay’s sea area.3-25μm plankton components were filtered and amplified18SrDNA variable region sequences from filtered samples were analyzed by DGGEtechnique. The results show that the main eukaryotic nanoplankton in the sea areaincluded Dinozoa, Ciliophora, Euglenozoa, Haptophyta, Ochrophyta,Labyrinthulomycota, Cryptista, Choanozoa, Amoebozoa and Cercozoan. The amountof Dinozoa and Ciliophora was the highest at41%and38.2%, respectively. Thecluster analysis based on mensal DGGE bands shows that the plankton communityfrom May to November2009is similar in the diversity of eukarytic nanoplankton.According to the studied results on scallop AVNV, Skeletonema costatum was takento be a vector of transmission of AVNV, and it distributed during the period of scallopmassive death. However, the role of Dinozoa and Ciliophora during the transmissionof AVNV has yet to be determined.In order to study the diversity of eukaryticplankton in the sea area for scallopculture of Sanggou Bay, seawater samples were collected in September2010fromRongcheng Sanggou Bay’s sea area.0.22-3μm and3-25μm plankton componentswere filtered and amplified18S rDNA variable region sequences from filteredsamples were analyzed by Clones libraries and PCR-RFLP. The results show that themain eukaryotic picoplankton in the sea area included Telonemia、Ochrophyta、Myzozoa、Haptophyta、Cryptista、Ciliophora、Choanozoa、Chlorophyta、Cercozoa、Opalozoa、Labrinthulomycota and Katablepharidophyta. the main eukaryoticnanoplankton in the sea area included Telonemia、Ochrophyta、Haptophyta、Cryptista、 Ciliophora、Chlorophyta、Choanozoa and Cercozoa. Ochrophyta、Ciliophora andChlorophyta were the main species in both communities. Skeletonema costatum wastaken to be a vector of transmission of AVNV, and it distributed in the nanoplanktoncommunity of Sanggou Bay.In order to study the diversity of eukarytic picoplankton in the sea area for scallopculture, and clarify the relationship between eukaryotic nanoplankton andtransmission of zhikong scallop (Chlamys farreri) acute viral necrosis virus (AVNV),seawater samples were collected monthly from May2010to April2011, fromRongcheng Sanggou Bay and Qingdao Liuqing River Bay’s sea area.0.22-3μmplankton components were filtered and amplified18S rDNA variable regionsequences from filtered samples were analyzed by DGGE technique. The results showthat the diversity of picoplankton in the sea area for Sanggou Bay is higher thanLiuqing River Bay. The Samples on summer (July, August, September) is significantdifferent from other manthes in Sanggou Bay. In Liuqing River Bay, the diversity ofpicoplankton on different monthes are distinct. The research shows that theenvironment of Sanggou Bay is better for scallop culture.