| Rice blast, caused by the fungus Magnaporthe oryzae, is one of the most severe diseases in rice production that significantly decrease the yields and quality of rice. However, the microarray-based expression profiles of rice genes in response to Magnaporthe oryzae and the molecular mechanisms of resistance genes remain largely unknown. This study investigated the blast resistance response of japonica rice variety Yunyin treated with the isolate Sichuan-43. The seedlings of Yunyin exhibited strong blast resistance compared with those of Lijiangxintuanheigu. The expression profile of Yunyin treated with Sichuan-43was generated using whole-genome microarray analysis. The resistance-related genes were screened from the large number genes based on the bioinformatics analysis. Gene Ontology classification and pathway enrichment analysis indicated that the differentially expressed genes, further build up the diagram of the various types of gene network regulation. Through systematic bioinformatic analysis of the microarray, we obtained a transcriptome map of Yunyin, which proved valuable in elucidating its blast resistance mechanisms. It enhance the current understanding of the effects of rice blast resistance genes at the transcription level, to further explore Yunyin’s molecular mechanism of blast resistance. To validate microarray data sets and investigate gene expression patterns, we analyzed the expression of some genes using real-time PCR Construction of RNA expression vector, preparation of the RNA probes for a candidate gene. |
