Effect Of Transgenic Human Lactoferrin Goats’ Milk On Metabolic Function In Rats

In order to estimate the safety of transgenic human lactoferrin (hLF) goats’milk, we have estabilished an experimental model, which influence the growth, metabolism, partial immunity and microstructure of intestinal tract in rats.1Determination of recombinant human lactoferrin expressed in goats’milkSix lactating transgenic hLF Boer goats and six wild type normal Boer goats were randomly selected and gathered milk in lactating day3,7,14,28; these milk were centrifuged at4℃,3500rpm for10min to remove milk fat and collected skim milk. Total milk protein concentration was judged by Bradford method, and then distinguished the different protein in skim milk by SDS-PAGE, analyzed the expression of lactoferrin in those milk by Western blot. The result indicated that LF was expressed high in colostrum, and the concentration of LF in transgenic goats’ milk is higher than in wild type normal goats’milk in each lactation period.2Effect of transgenic human lactoferrin goats’milk on metabolic function in ratsFifteen healthy SD female rats and fifteen healthy male rats weighted about100g were randomly distributed into three groups. Transgenic hLF goats’milk (protein concentration at0.93g·mL-1), at lml/100g of body weight, or an equal concentration and volume of wild type normal goats’milk or an equal volume of sterile pyrogen-free physiological saline was administered to rats two times a day by gavage. Three groups were provided a standard rodent chow and an ad libitum supply of water. After15days of gavage, all rats were killed by collecting living body blood in neck. Blood glucose (BG), Glutamic-oxalacetic transaminase (GOT), Glutamic-pyrutic transaminase (GPT), Serum urea nitrogen (SUN), Serum creatinine (Scr), Total cholesterol (TC) level of serum were detected by Glucose oxidase-peroxidase end Colorimetry, Reitman-Frankel method, Urease method, In addition to the protein method, CE-COD-POD Colorimetry. The level of insulin, glucagon, triiodothyroxine (T3), thyroxin (T4) detected from serum were determined by radioimmunoassay (RIA). The result indicated that the Average daily gain of the female and male rats which were infused with transgenic hLF goats’milk increased in evidence compared to the Physiological Saline group (P<0.01) and the normal milk group (P<0.05). However, there were no difference between the normal milk group and the Physiological Saline group. And the other measurements and biochemical examination of the recombinant milk group and the normal milk group didn’t change significantly compared to the Physiological Saline group (P>0.05), moreover, no obvious change were observed between the recombinant and wild type group. Overall, the result of the present study demonstrates that transgenic hLF goats’milk can enhance rats growth performance but has no effect on its’metabolism by gavage administration in short time.3Effect of transgenic human lactoferrin goats’milk on intestinal tract and partial immunity parameter in ratsSecreting type Immunoglobulin A (SIgA) detected from intestine mucous and Interleukin-2(IL-2) detected from serum were detected by Radioimmunoassay (RIA); The microstructure of intestine mucous was observed by hematoxylin eosin (H.E) and counted the length of duodenum villous. The result showed that the milk expressing human lactoferrin hadn’t effect on the level of IL-2and SIgA compared to control groups, but increased the length of rats’duodenum villous. Overall, the result indicated that the transgenic hLF goats’milk has no obviously negative effect on partial immunity parameter which play an important role in immune system, but can improve the growth performance of rats’intestine mucous and facilitate better nutrient digestion and absorption.