The Effects Of Glycerol-Enriched Probiotics On Rumen Metabolism And Blood Biochemical Indexes In Goats

Nity percent of the ruminants depended on gluconeogenesis to satisfy the needs of glucose. Because of the limited ability of ruminant directly absorbing and utilizing glucose, the animals likely occurr energy metabolism disorders. Glycerol is closely related with glucose metabolism, it is an important glucogenic substance. Glycerol always metabolized in the liver, to produce more energy for the body needs. So it can be added to the diet of ruminants, can effectively solve the animal energy metabolism problems. However, the impurities in crude glycerol always lead to disease. Glycerol-enriched probiotics can slove this problem. It has many advantages such as containing beneficial microbial strains, low costs, pollution-free and can be used widely.The objectives of this study were to investigate the transformation of glucose to glycerol by specific probiotics producing microbical feed additives which can exert double effects of glycerol and probiotics at the same time. Further more, the investigation was to study the effect of dietary supplementation of glycerol-enriched probiotics on rumen metabolism and hematologic value, so that to provide an experimental basis for application of enriched probiotics in ruminants industry.Experiment1Study on the preparation of glycerol-enriched probiotics and its stabilization in storageThe differences on the yield of glycerol, the growth of probiotics between different culture apparatus fermentation tank and conical flask under certain conditions were researched. Various conditions on yield of glycerol, growth of probiotics were estimated when product was stored. The result showed that the glycerol concent was58.6g/L,and the population of probitic was6.89×1010cfu/mL in conical flask, while42.3g/L glycerol and5.37×101cfu/mL in fermentation tank.Experiment2Effects of glycerol-enriched probiotics supplementation on rumen metabolism in goats An experiment was conducted with eight rumen cannulated goats using4X4Latin square design with4periods to determine the effects of glycerol-enriched probiotics supplementation on rumen fermentation in goats. The4supplemental levels of glycerol-enriched probiotocs for goats were0mL/d (control),30mL/d (LSP),60mL/d (MSP) and90mL/d (HSP), respectively. Rumen fluid was taken via the rumen fistula at the last day of each period. The concentrations of propionate, the ratio of acetate to propionate and MCP in groups of HSP were greater (P<0.05) when compared with control.The concentrations of ammonia-N and rumen pH were lower in groups of HSP compared to control (P<0.05). Compared to control, goats supplemented with liquid glycerol in the current experiment caused an increase in concentrations of butyrate and MCP, and improved the rumen fermentation. The concentrations of acetate were not significantly affected by glycerol-enriched probiotics supplementation (P>0.05), but tended to decrease compared to the control.Experiment3Effects of glycerol-enriched probiotics supplementation on blood biochemical indexes in goatsAn experiment was conducted with eight rumen cannulated goats using4×4Latin square design with4periods to determine the effects of glycerol-enriched probiotics supplementation on hematologic performence in goats. The4supplemental levels of glycerol-enriched probiotocs for goats were0mL/d (control),30mL/d (LSP),60mL/d (MSP) and90mL/d (HSP), respectively. Blood samples were taken from the jugular veins at the last day of each period for examination of blood cells. The results showed that the numbers of total red blood cell, total white blood cell, lymphocytes and neutrophilic granulocytes were not significantly affected by glycerol-enriched probiotics supplementation (P>0.05). The serum cholesterol, triglyceride and blood urea nitrogen were not affected by glycerol supplementation (P>0.1). Compared to the control, glycerol-enriched supplementation increased the concentrations of serum total protein and blood glucose (P<0.05). The plasma total protein in the treatments of LSP, MSP and HSP increased by3.94%,6.33%and8.06%, respectively, and blood glucose concentration increased by7.47%,14.29%and15.7%.