| Canine parvovirus(CPV) is a significant pathogen for dogs and the associated animals, which causes an acute haemorrhagic diarrhoea and sometimes fatal myocarditis in young dogs. In this paper, the genotypes of CPV isolates in China were characterized.Eleven parvovirus strains were isolated from the feces of30dogs from different time and different regions. The11isolates were designated CPV through a series of systematic identification assays, such as physicochemical, biological and molecular virology assay, and so on. These strains can grow in F81cell line and produce cytopathic effects with the characteristics of CPV, swelling, rounding, broken, droping. The strains resist the chloroform treatment and are stable in pH3.0and56℃, but are suppressed by5-IUDR. The viruses can agglutinate RBC of pig, horse, cat, but not of human, chicken, guinea pig. The positive antibody against CPV can inhibit HA of these isolates. The results implied that CPV exists in canine population in China.To examine the genotypes of the11CPV isolates during2008-2010in our lab, the CPV VP2genes were amplified and sequenced. The sequences of these isolates were compared with those of the reference strains for the analysis of specific and important nucleotides. Two isolates were confirmed to be CPV-2a, and seven isolates were CPV-2b. Furthermore, two new mutants of CPV-2a were found, which had almost the same amino acid sequences as conventional CPV-2a. CPV09-04and CPV10-05possessed a G→S substitution at amino acid residue300. At present, the exactly biological importance of the mutants remains unknown. The total nucleotide homologies of VP2genes among the CPV isolates to the references are beyond98%.The CPV isolates in China didn’t obviously form a typical cluster of China. It provides basically important information for the prevention and treatment of CPV. |
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