Research On Tissue Culture Propagation Technique Of Rose Rugosa ’Purple Hibiscus’

As Chinese traditional flower, Rosa rugosa belongs to the deciduous shrub ofRosa Rosaceae. It is not only excellent landscaping and soil conservation plants, butalso precious Chinese herbal medicines, as well as important raw material for the foodindustry and perfumery industry. Rosa rugosa is important ornamental plantsbreeding resource of Rosa, its genome containing aromatic, cold resistance, saltresistance and other quality traits. With the social progress and technologicaldevelopment, the development of Rose rugosa production has become popular inmany areas. As to the Rose rugosa market with growing demand, traditional Roserugosa breeding methods like grafting, cuttings, layering-based and breeding meanshaven’t met market demand. Therefore, it has a very important scientific value andsocial significances to explore the use of the Micropropagation modernbiotechnological tools to vigorously develop Rose resources, improve the speed ofRosa rugosa propagation, breed new varieties and increase production, make Roserugosa tissue culture onto the factory road.In recent years, plant tissue culture propagation technology developed veryquickly, has formed a relatively complete set of technical system, having a broaddevelopment prospects. The plantlets industry has been established at domestic andabroad, conducting large-scale and commercial production, which is the mosteffective and maximum to use the plant tissue culture. At present,130families, morethan1500kinds can regenerate through tissue culture, such as Dianthus caryophyllus,Rose Chinensis, Rhododendron, Lilium brownii.In order to meet the development needs of the Rose rugosa market, increase thepropagation speed, solve the technical problems of high-quality varietiesmicropropagation, breed new varieties and increase production, relieve the difficultiesthat traditional Rose rugosa propagation mode can’t meet the needs of market. Rosarugosa ’Purple Hibiscus’ stem with axillary bud was used as explants to studysystematically the tissue culture in this article.There are many systematic and detailed studies in the article including screeningof the explants sterilization ways, the browning phenomenon of explants and thecontrol of browning methods, screening of the primary medium, screening of theproliferation medium, screening of the rooting medium, the effect of plant growthregulating substances on the various stages of tissue culture, the effect of lightconditions on tissue culture proliferation, the effect of numbers of following passage on tissue culture proliferation, PPO activity and rooting status of tissue culture underdifferent pH conditions, the effect of domestication process and transplanting mediumon survival rate of transplants. The results indicate that:1. The optimal germicidal treatment program of Rose rugosa ’purple Hibiscss’explant seedlings is T5treatment, that is:75%C2H5OH1minute+0.1%HgCl2(addtween-20)10minutes.2. The optimal primary medium for primary culture of Rose rugosa ’purpleHibiscss’ is MS＋6-BA0.5mg/L＋NAA0.1mg/L＋GA30.4mg/L.3. The browning degree of different bud locations of Rose rugosa ’purpleHibiscss’ is different, but there is a "V"-type change in the average browning degreeand the peak amplitude is the fourth bud. The optimal browning inhibitor in primaryculture and rooting culture is1.5g/L activated carbon (AC), and the optimal browninginhibitor in proliferation culture is0.2g/L ascorbic acid (Vc).4. There are prominent promotion effect of6-BA with suitable density to thehight and the proliferation coefficient of Rose rugosa ’purple Hibiscss’ plantlets, the6-BA is the dominating effect factor to proliferation of plantlets,but NAA is not thedominating effect factor, and GA3is the important effect factor to proliferation ofplantlets. The optimal proliferation medium of Rose rugosa ’purple Hibiscss’ plantletsis MS＋6-BA1.0mg/L＋NAA0.05mg/L＋GA30.8mg/L.5. The minimum intensity of illumination for the normal growth of plantlets is2000LUX. The maximum intensity of illumination which ensure plantlets withrelatively higher proliferation coefficient is3000LUX.The optimal intensity ofillumination scope for tissue culture of Rose rugosa ’purple Hibiscss’ plantlets is2000~3000LUX.6. The proliferation coefficient of Rose rugosa ’purple Hibiscss’ plantlets begin todecline from the6th proliferation culture. With the increase of proliferation culturefrequency the proliferation coefficient of plantlets continue to decline. The Roserugosa ’purple Hibiscss’ plantlets should be renewed regularly during commercialproduction.7. Both PPO activity and rooting index of Rose rugosa ’purple Hibiscss’ plantletshad significant correlation with PH; the optimal pH of PPO was6.5, while the optimalpH of vitro rooting was5.5.8. The effect of NAA on rootage of Rose rugosa ’purple Hibiscss’ plantlets issignificant. IBA has smaller effect than NAA on rootage of Rose rugosa ’purple Hibiscss’ plantlets.The optimal rooting medium of Rose rugosa ’purple Hibiscss’plantlets is1/2MS+NAA0.2mg/L+IBA0.5mg/L+AC1.5g/L.9.Acclimation treatment is the indispensable process before transplant of Roserugosa ’purple Hibiscss’ plantlets. The optimal matrix of the transplant of Rose rugosa’purple Hibiscss’ plantlets is pearlite: vermiculite:grass peat=1:2:1...