Studies On The Testis, Vas Deferens, Spermatophore Histology And Testis’ Annual Variation Of Procambarus Clarkii

There are scarce researches about the male reproductive system of Procambarus clarkii. This study aims to get some festures of the male reproductive system of P.clarkii. The ultrastructure of the vas deferens was stuied by using transmission electron microscopy, the microstructure of testis、vas deferens and spermatophore of P.clarkii were studied by using paraffin method, and the annual variation of the testis was investigated. The results are as follows:1. Testis are comprised by many spermatogenicaciniand collecting ducts whichstructure is like grapes;The germ cells in the spermatogenic acini are transported to the vas deferens by through the collecting ductafter matured;2. P.clarkiihas a right and a left vas deferens, the right vas deferens was half time longer than the left, the right vas deferens has a larger diameter, its curling degree and inclusion content are materially higher than the left, secretory cells of the right vas deferens has more abundant endoplasmic reticulum. The vas deferens candivideinto anterior middle、posterior section and a male gonopores acording to the difference of its morphous. Anterior and middle vas deferens can secrete materials for the formation of the spermatophore. The posterior section can store and ejaculate spermatophore. Male gonopores are the outcome of the vas deferens.3. The shape of spermatophore is elongated capsule or ellipsoidal.The spermatophore is composed of sperm mass, spermatophore matrix and two layer of spermatophore wall, sperm mass is in one side of the spermatophore.4. Male P.clarkiihas two maturity period that the most part of spermatogenic acini in the testis are at spermatid stage in its annual variation, one is from May to August and the other one is at October.